The overall goal of the program is to develop molecular biology skills necessary to continue as an independent researcher in the field of craniofacial wound healing. The investigator has an established interest in defining the molecular basis of scarless wound healing. The research plan involves laboratory research activities addressing the problem of scarless wound healing.
Four aims are proposed: 1) to isolate, clone, and sequence genes that are over-expressed in scarless-healing wounds compared to scar-wounds in the rat model of fetal wound healing (""""""""scarless repair-specific"""""""" genes); 2) to obtain the full-length cDNAs of genes that are differentially expressed in fetal scarless wounds compared with postnatal scar wounds, unwounded fetal skin, and unwounded postnatal skin; 3) to identify and locate the cells expressing scarless repair-specific genes within scarless fetal rat wounds; and 4) to determine the effects of over-expression and under-expression of a scarless repair-specific gene on fetal and postnatal fibroblasts. A long-term goal is to apply these skills to gene therapy for the treatment of craniofacial defects. A laboratory research focus will be enhanced by formal courses in the biological sciences and statistics. An ethics for health providers course will also be included. As well, the applicant will attend the Workshop in Clinical Research at UCLA. Finally, the applicant will spend 15 to 20 percent of time treating patients.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08DE000463-06
Application #
6917256
Study Section
Special Emphasis Panel (ZDE1-WG (41))
Program Officer
Hardwick, Kevin S
Project Start
2001-08-15
Project End
2006-07-31
Budget Start
2005-08-01
Budget End
2006-07-31
Support Year
6
Fiscal Year
2005
Total Cost
$126,630
Indirect Cost
Name
Stanford University
Department
Surgery
Type
Schools of Medicine
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Kong, Wuyi; Li, Shaowei; Longaker, Michael T et al. (2007) Cyclophilin C-associated protein is up-regulated during wound healing. J Cell Physiol 210:153-60
Kong, Wuyi; Longaker, Michael T; Lorenz, H Peter (2004) Cyclophilin C-associated protein is a mediator for fibronectin fragment-induced matrix metalloproteinase-13 expression. J Biol Chem 279:55334-40
Kong, Wuyi; Longaker, Michael T; Lorenz, H Peter (2003) Molecular cloning and expression of keratinocyte proline-rich protein, a novel squamous epithelial marker isolated during skin development. J Biol Chem 278:22781-6
Yang, George P; Lim, Ivor J; Phan, Toan-Thang et al. (2003) From scarless fetal wounds to keloids: molecular studies in wound healing. Wound Repair Regen 11:411-8