CANDIDATE. Dr. Webb has drawn on her academic achievement and diverse training and experience in clinical and basic science to develop an independent research program. She has developed laboratory skills in molecular and cellular biology and has acquired computational skills in bioinformatics and systems biology. Additionally, her clinical training in Medical Genetics provides her with a strong background in inherited metabolic diseases. Recognition of Dr. Webb's potential as an investigator is reflected in her current appointment of Assistant Professor (tenure-track position, Investigator Track) in the Departments of Genetics and Genomic Sciences (primary) and Pediatrics (secondary) at the Icahn School of Medicine at Mount Sinai. She has been allocated >75% protected research time. Dr. Webb's accomplishments include several first- author publications and additional co-authored manuscripts in high quality journals. CAREER DEVELOPMENT. Dr. Webb's overall long-term career goal is to lead a meaningful and sustainable research program that will allow her to remain a well-established and independent investigator in the fields of metabolism, developmental genetics, and systems biology. These goals will be accomplished through the mentorship, collaboration, and didactic mechanisms outlined in this application. INSTITUTIONAL ENVIRONMENT. The Department of Genetics and Genomic Sciences and the Icahn Institute for Genomics and Multiscale Biology at the Icahn School of Medicine at Mount Sinai is the ideal setting for carrying out the work described in this application. Faculty in the Department are experts in the identification, treatment, and study of inborn errors of metabolism. Additionally, members of the Icahn Institute are world-renown in the ability to use systems biology and computational techniques to study disease. RESEARCH PROJECT. Mitochondrial aminoacyl-tRNA synthetases (mt-ARSs) are essential for protein synthesis in mitochondria, and disruption of mt-ARS function represents a new class of mitochondrial disease. A novel disorder caused by recessive, single nucleotide variants in methionyl-tRNA synthetase 2 (MARS2) associated with developmental delay, growth failure, and sensorineural hearing loss has been identified by the candidate. The proposed project will utilize an integrative approach, combining both wet-laboratory and in silico techniques, to further study mt-ARS disorders. Pathogenic variants in MARS2 will be further characterized biochemically by assessing aminoacylation activity, mitochondrial protein synthesis, and mitochondrial oxygen consumption. To better understand the variable phenotypes and tissue specificity of different mt-ARS disorders, an in vitro model system of several mt-ARS disorders will be developed using CRISPR/Cas9 technologies. RNA-seq data will be generated and network analysis will be completed to identify key drivers and developmental pathways involved in mt-ARS disorders.

Public Health Relevance

Although rare, mitochondrial disease may cause significant morbidity and mortality due to multi-organ system involvement. A specific class of mitochondrial disease caused by mutations in mitochondrial aminoacyl-tRNA synthetases has recently been discovered. The proposed work aims to better understand the pathophysiology or disease process of this class of disorders.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08HD086827-02
Application #
9356529
Study Section
National Institute of Child Health and Human Development Initial Review Group (CHHD)
Program Officer
Krotoski, Danuta
Project Start
2016-09-20
Project End
2021-06-30
Budget Start
2017-07-01
Budget End
2018-06-30
Support Year
2
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Icahn School of Medicine at Mount Sinai
Department
Genetics
Type
Schools of Medicine
DUNS #
078861598
City
New York
State
NY
Country
United States
Zip Code
10029
Bruni, Francesco; Di Meo, Ivano; Bellacchio, Emanuele et al. (2018) Clinical, biochemical, and genetic features associated with VARS2-related mitochondrial disease. Hum Mutat 39:563-578
Webb, Bryn D; Metikala, Sanjeeva; Wheeler, Patricia G et al. (2017) Heterozygous Pathogenic Variant in DACT1 Causes an Autosomal-Dominant Syndrome with Features Overlapping Townes-Brocks Syndrome. Hum Mutat 38:373-377
Lake, Nicole J; Webb, Bryn D; Stroud, David A et al. (2017) Biallelic Mutations in MRPS34 Lead to Instability of the Small Mitoribosomal Subunit and Leigh Syndrome. Am J Hum Genet 101:239-254