The control of hematopoiesis in the human fetal liver will be investigated using the in vitro methyl cellulose culture system. Techniques of tissue culture, cell biology, immunology and membrane chemistry will be employed to answer key unsolved problems in our understanding of normal and abnormal hematopoisis.
Specific aims of these studies will be: 1) To use positive and negative selection techniques on fetal liver cells, to enrich human BFU-E, CFU-GM, and CFU-GEMM; 2) to analyze the cell interactions which control the differentiation of these hematopoietic progenitors; 3) to determine which, in any, of the cell interaction molecules known to be involved in immune interactions are found on purified hematopoietic cells. If they are present, whether these molecules act as molecular signals between hematopoietic and regulatory cells will be determined; and 4) to utilize the enriched progenitor population as an immunogen to raise antibodies to membrane determinants unique to progenitor cells. The information obtained by these studies should greatly improve our understanding of normal hematopoiesis as well as the clinical disease states of aplastic anemia, hypoplastic anemia and lymphocytosis with cytopenias.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08HL001949-05
Application #
3082368
Study Section
(SRC)
Project Start
1986-07-01
Project End
1989-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
5
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
Schools of Medicine
DUNS #
791277940
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109