Abstract

The objectives are (1) to characterize the type of cell that produces erythropoietin in the kidney, and (2) to determine the gene sequences that control transcription and nuclear trans-acting factors that interact with these sequences. The methods include in vitro transcription systems using whole nuclei and nuclear extracts, transient expression analysis of transfected cell cultures, and DNase footprinting.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08HL002167-04
Application #
3082556
Study Section
Research Manpower Review Committee (MR)
Project Start
1990-08-01
Project End
1995-07-31
Budget Start
1993-08-15
Budget End
1994-07-31
Support Year
4
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
Thomas Jefferson University
Department
Type
Schools of Medicine
DUNS #
061197161
City
Philadelphia
State
PA
Country
United States
Zip Code
19107

  Publications

Schuster, S J; Caro, J (1993) Erythropoietin: physiologic basis for clinical applications. Vox Sang 65:169-79
Schuster, S J; Koury, S T; Bohrer, M et al. (1992) Cellular sites of extrarenal and renal erythropoietin production in anaemic rats. Br J Haematol 81:153-9
Konkle, B A; Schuster, S J; Kelly, M D et al. (1992) Plasminogen activator inhibitor-1 messenger RNA expression is induced in rat hepatocytes in vivo by dexamethasone. Blood 79:2636-42