Fluid secretion by airway epithelium is important for normal lung development, mucocilliary clearance, and in disease processes such as asthma or cystic fibrosis. This grant is designed to study the controls of ion transport across airway epithelium during fetal development using electrophysiological, morphological, and genetic approaches.
The specific aims are as follows: 1) To establish and characterize primary cell cultures of tracheal epithelium from rabbit and human fetuses of different gestational periods; 2) To search for molecules that regulate fluid transport during the transition from fetal to adult life; and 3) To study the signal transduction processes between cell surface receptors and ion channels in fetal tissues and to contrast the fetal with adult mechanisms.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08HL002188-02
Application #
3082569
Study Section
(SRC)
Project Start
1988-08-01
Project End
1993-07-31
Budget Start
1989-08-01
Budget End
1990-07-31
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Type
Schools of Medicine
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
McGrath, S A; Basu, A; Zeitlin, P L (1993) Cystic fibrosis gene and protein expression during fetal lung development. Am J Respir Cell Mol Biol 8:201-8
Egan, M E; Wagner, M H; Zeitlin, P L et al. (1992) Modulation of ion transport in cultured rabbit tracheal epithelium by lipoxygenase metabolites. Am J Respir Cell Mol Biol 7:500-6
Wiebke, J L; Montrose-Rafizadeh, C; Zeitlin, P L et al. (1992) Effect of hypoxia on endothelin-1 production by pulmonary vascular endothelial cells. Biochim Biophys Acta 1134:105-11
Zeitlin, P L; Lu, L; Rhim, J et al. (1991) A cystic fibrosis bronchial epithelial cell line: immortalization by adeno-12-SV40 infection. Am J Respir Cell Mol Biol 4:313-9
Trapnell, B C; Zeitlin, P L; Chu, C S et al. (1991) Down-regulation of cystic fibrosis gene mRNA transcript levels and induction of the cystic fibrosis chloride secretory phenotype in epithelial cells by phorbol ester. J Biol Chem 266:10319-23
Lu, L; Zeitlin, P L; Guggino, W B et al. (1989) Gene transfer by lipofection in rabbit and human secretory epithelial cells. Pflugers Arch 415:198-203
Hwang, T C; Lu, L; Zeitlin, P L et al. (1989) Cl- channels in CF: lack of activation by protein kinase C and cAMP-dependent protein kinase. Science 244:1351-3