The initial phase of research will investigate the calcium channels in T- lymphocytes. Studies have shown that activation of T lymphocytes involves an increase of intracellular free calcium. While the initial rise in intracellular calcium is due to a release of intracellular stores mediated by inositol 1,4,5 trisphosphate (InsP3), evidence suggest that the sustained rise in calcium is due to a transmembrane influx of calcium. The voltage insensitive calcium channel responsible for this sustained influx is not yet fully characterized and is the subject of this proposal. Patch clamp techniques will be utilized to demonstrate regulation by InsP3. Single channel recordings of cell-attached and inside-out patches will be compared to whole cell studies. Monovalent and divalent cation permeability will be determined. The effect of specific blockers on the calcium channel current will also be studied. During the second phase of research, the properties determined by single channel recordings will be compared to purified cerebellar InsP3 receptors reconstituted in lipid bilayers.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Physician Scientist Award (K11)
Project #
5K11AI001201-02
Application #
2057338
Study Section
Allergy & Clinical Immunology-1 (AITC)
Project Start
1994-09-01
Project End
1996-07-31
Budget Start
1995-09-01
Budget End
1996-07-31
Support Year
2
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Stanford University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305