Our investigation will focus on genetic exchange among oral bacteria, especially among periodontopathic organisms. The transfer of genetic information between different organisms has been inferred from nucleotide sequence comparisons. The ecological benefits of genetic exchange include the expansion of metabolic versatility and resistance to hazardous environments. Some transformable bacteria have acquired antibiotic resistance by horizontal genetic exchange of fragments of chromosomal genes, such as penicillin-resistant penicillin-binding proteins in Streptococcus pneumoniae. The application of molecular biology techniques with oral microorganisms should permit the opportunity for an in vitro assessment of gene transfer.
The specific aim of our investigation is to understand the role of genetic exchange as a risk factor in human oral infections such as periodontal diseases. Little information exists on gene transfer between the bacterial flora associated with periodontal diseases. Among the periodontopathic organisms, T. denticola, P. gingivalis, and F. nucleatum are the focus of our present investigation. The recent development of a gene transfer system in T. denticola in Dr. Kuramitsu's laboratory now makes it feasible to study whether the genetic information from T. denticola can be transferred in vitro to P. gingivalis or to F. nucleatum. We are also planning to determine if the exchange of genetic information can occur between T. denticola and oral streptococci, since the latter forms the majority of gram positive early colonizers of the tooth surface. Both in vitro bacterial culture and biofilms will be used to evaluate gene transfer from T. denticola to different bacteria. Information obtained from this study could elucidate the potential role of gene transfer between oral bacteria in the etiology of periodontal diseases. Key words: Genetic exchange, biofilms, periodontal disease

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
5K16DE000158-14
Application #
6104536
Study Section
Project Start
1999-07-01
Project End
2000-06-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
14
Fiscal Year
1999
Total Cost
Indirect Cost
Name
State University of New York at Buffalo
Department
Type
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260
Krebs, Linda J; Wang, Xiaopeng; Nagy, Atilla et al. (2002) Bombesin and epidermal growth factor potentiate the effect of cytotoxic LH-RH analog AN-152 in vitro. Int J Oncol 21:1325-9
Krebs, Linda J; Wang, Xiaopeng; Nagy, Attila et al. (2002) A conjugate of doxorubicin and an analog of Luteinizing Hormone-Releasing Hormone shows increased efficacy against oral and laryngeal cancers. Oral Oncol 38:657-663
Rogers, J D; Scannapieco, F A (2001) RegG, a CcpA homolog, participates in regulation of amylase-binding protein A gene (abpA) expression in Streptococcus gordonii. J Bacteriol 183:3521-5
Krebs, L J; Wang, X; Pudavar, H E et al. (2000) Regulation of targeted chemotherapy with cytotoxic lutenizing hormone-releasing hormone analogue by epidermal growth factor. Cancer Res 60:4194-9
Malek, R; Fisher, J G; Caleca, A et al. (1994) Inactivation of the Porphyromonas gingivalis fimA gene blocks periodontal damage in gnotobiotic rats. J Bacteriol 176:1052-9
Dolce, C; Anguita, J; Brinkley, L et al. (1994) Effects of sialoadenectomy and exogenous EGF on molar drift and orthodontic tooth movement in rats. Am J Physiol 266:E731-8
Stephan, E B; Dziak, R (1994) Effects of genistein, tyrphostin, and pertussis toxin on EGF-induced mitogenesis in primary culture and clonal osteoblastic cells. Calcif Tissue Int 54:409-13
Grossi, S G; Zambon, J J; Ho, A W et al. (1994) Assessment of risk for periodontal disease. I. Risk indicators for attachment loss. J Periodontol 65:260-7
Winston, J L; Chen, C K; Neiders, M E et al. (1993) Membrane protein expression by Actinobacillus actinomycetemcomitans in response to iron availability. J Dent Res 72:1366-73
Sharma, A; Sojar, H T; Lee, J Y et al. (1993) Expression of a functional Porphyromonas gingivalis fimbrillin polypeptide in Escherichia coli: purification, physicochemical and immunochemical characterization, and binding characteristics. Infect Immun 61:3570-3

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