Abstract

In a successful wound healing event, endothelial cells adjacent to a site of mechanical wound must perceive that an injury has occurred; migrate into the region; proliferate to restore the flawed area; and finally, return to a nonproliferative state. This process involves the regulation of many genes. To elucidate these genes, an in vitro wound healing model using endothelial cell cultures, maintained on an artificial basement membrane of type IV collagen, is being developed. Differential display will be utilized to isolate and identify mRNAs that are regulated during this wound healing process. This approach, along with the use of an in vivo model of infective endocarditis, may provide insights into the disease mechanisms underlying the healing of injured or diseased heart valves which may become the site for the development of platelet vegetations. Differential gene regulation during wound healing of this relatively avascular, collagenous valve tissue, may play a key role determining whether repair or vegetation formation occurs.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K16)
Project #
2K16DE000270-06
Application #
5210057
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Luger, Nancy M; Mach, David B; Sevcik, Molly A et al. (2005) Bone cancer pain: from model to mechanism to therapy. J Pain Symptom Manage 29:S32-46
Sevcik, Molly A; Luger, Nancy M; Mach, David B et al. (2004) Bone cancer pain: the effects of the bisphosphonate alendronate on pain, skeletal remodeling, tumor growth and tumor necrosis. Pain 111:169-80
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Sabino, Mary Ann C; Honore, Prisca; Rogers, Scott D et al. (2002) Tooth extraction-induced internalization of the substance P receptor in trigeminal nucleus and spinal cord neurons: imaging the neurochemistry of dental pain. Pain 95:175-86
Tran, S D; Rudney, J D; Sparks, B S et al. (2001) Persistent presence of Bacteroides forsythus as a risk factor for attachment loss in a population with low prevalence and severity of adult periodontitis. J Periodontol 72:1-10
Herzberg, M C (2001) Coagulation and thrombosis in cardiovascular disease: plausible contributions of infectious agents. Ann Periodontol 6:16-9
O'Brien, T P; Roszkowski, M T; Wolff, L F et al. (1996) Effect of a non-steroidal anti-inflammatory drug on tissue levels of immunoreactive prostaglandin E2, immunoreactive leukotriene, and pain after periodontal surgery. J Periodontol 67:1307-16
Reeh, E S; Ross, G K (1994) Tooth stiffness with composite veneers: a strain gauge and finite element evaluation. Dent Mater 10:247-52
Skopek, R J; Liljemark, W F (1994) The influence of saliva on interbacterial adherence. Oral Microbiol Immunol 9:19-24

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