I am a Professor of Medicine at the University of California San Francisco (UCSF). I am a pulmonary and critical care medicine specialist with a clinical and translational research focus in HIV-associated pulmonary diseases, and I am committed to training the next generation of researchers in this field. For this K24 renewal, my career goals and research objectives are to: (1) continue to provide comprehensive mentoring and research support to T32/F32 fellows and K Assistant Professors on research and career development and focused mentoring to other junior investigators on specific research projects. A special emphasis will be assisting my K mentees to make the challenging transition to full independence (e.g., R01 award); (2) expand my Distance Mentoring and Mentoring the Mentor experience; (3) join a new UCSF initiative and assist under-represented minority and women faculty obtain their own K24 awards; (4) continue my research focus on HIV-associated opportunistic pneumonias, the lung microbiome, and chronic obstructive pulmonary disease (COPD) and expand my current research to study the lung microbiome that persists after pneumonia and declines in lung function and development COPD. Our central hypothesis is that post-pneumonia changes in the lung microbiome are associated with declines in lung function and the development of COPD in HIV- infected persons.
Aim 1 : To compare the composition of the lung microbiome at baseline and 3-months and 1- year after acute pneumonia treatment using a standardized phylogenetic microarray (PhyloChip).
Aim 2 : To compare the structure of the lung microbiome at baseline and 3-months and 1-year after acute pneumonia treatment using high-throughput DNA sequencing (Illumina MiSeq).
Aim 3 : To correlate the lung microbiome composition and structure with changes in lung function and development of COPD in this cohort. To address these aims, we will conduct a longitudinal study of 25 HIV-infected Pneumocystis pneumonia (PCP) subjects and 25 HIV-infected TB subjects, and we will perform research bronchoscopy at 3-months and 1-year after the completion of pneumonia treatment. We will compare the lung microbiome in these individuals at the time of pneumonia to the lung microbiome after the completion of treatment using PhyloChip and MiSeq and will correlate microbiome composition and structure with lung function and chest computed tomography measurements.

Public Health Relevance

This proposal studies the convergence of four of the greatest causes of morbidity and mortality in the world. Worldwide, HIV/AIDS, pneumonia, TB, and COPD are among the top 10 causes of death. The proposed studies have the potential for advancing our knowledge of the link between HIV-associated pneumonias, including TB, and COPD.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Midcareer Investigator Award in Patient-Oriented Research (K24)
Project #
5K24HL087713-09
Application #
9236079
Study Section
Special Emphasis Panel (ZHL1)
Program Officer
Colombini-Hatch, Sandra
Project Start
2006-09-30
Project End
2018-12-31
Budget Start
2017-01-01
Budget End
2017-12-31
Support Year
9
Fiscal Year
2017
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94118
Musisi, Emmanuel; Matovu, Denis Kasozi; Bukenya, Andrew et al. (2018) Effect of anti-retroviral therapy on oxidative stress in hospitalized HIV-infected adults with and without TB. Afr Health Sci 18:512-522
Gingo, Matthew R; Nouraie, Mehdi; Kessinger, Cathy J et al. (2018) Decreased Lung Function and All-Cause Mortality in HIV-infected Individuals. Ann Am Thorac Soc 15:192-199
Qin, Shulin; Clausen, Emily; Nouraie, Seyed Mehdi et al. (2018) Tropheryma whipplei colonization in HIV-infected individuals is not associated with lung function or inflammation. PLoS One 13:e0205065
Li, Yijia; Nouraie, Seyed Mehdi; Kessinger, Cathy et al. (2018) Factors Associated With Progression of Lung Function Abnormalities in HIV-Infected Individuals. J Acquir Immune Defic Syndr 79:501-509
Tipton, Laura; Cuenco, Karen T; Huang, Laurence et al. (2018) Measuring associations between the microbiota and repeated measures of continuous clinical variables using a lasso-penalized generalized linear mixed model. BioData Min 11:12
Tipton, Laura; Müller, Christian L; Kurtz, Zachary D et al. (2018) Fungi stabilize connectivity in the lung and skin microbial ecosystems. Microbiome 6:12
Farr, Katherine; Ravindran, Resmi; Strnad, Luke et al. (2018) Diagnostic performance of blood inflammatory markers for tuberculosis screening in people living with HIV. PLoS One 13:e0206119
Worodria, William; Chang, Emily; Andama, Alfred et al. (2018) Predictors of Mortality Among Hospitalized Patients With Lower Respiratory Tract Infections in a High HIV Burden Setting. J Acquir Immune Defic Syndr 79:624-630
Walter, Nicholas D; Moore, Camille M; Kayigire, Xavier A et al. (2018) Does discovery of differentially culturable M tuberculosis really demand a new treatment paradigm? Longitudinal analysis of DNA clearance from sputum. BMC Infect Dis 18:293
Morris, Alison; Fitzpatrick, Meghan; Bertolet, Marnie et al. (2017) Use of rosuvastatin in HIV-associated chronic obstructive pulmonary disease. AIDS 31:539-544

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