Abstract

1. Separate M. leprae baccili from infected armadillo tissues and distribute the purified bacilli to investigators upon request. 2. Purify and supply phenolic glycolipid I and dimycocerosylphthiocerol from M. leprae-free supernatant material derived from homogenization of infected armadillo tissue. 3. Synthesize and provide the Neoglycoconjugates based on phenolic glycolipid I for the purpose of the serological diagnosis of leprosy. 4. Purify and provide the native lipoarabinomannan and lipomannan of M. leprae and M. tuberculosis. 5. Isolate the peptidoglycan-associated proteins of M. leprae. 6. Isolate, purify and characterize other native protein antigens and sub- cellular entities from M. leprae.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research and Development Contracts (N01)
Project #
N01AI005074-010
Application #
2294713
Study Section
Project Start
1990-07-01
Project End
1995-06-30
Budget Start
1994-03-28
Budget End
1995-06-30
Support Year
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Colorado State University-Fort Collins
Department
Public Health & Prev Medicine
Type
Schools of Veterinary Medicine
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523

  Publications

Cho, S N; Cellona, R V; Villahermosa, L G et al. (2001) Detection of phenolic glycolipid I of Mycobacterium leprae in sera from leprosy patients before and after start of multidrug therapy. Clin Diagn Lab Immunol 8:138-42
Schorey, J S; Li, Q; McCourt, D W et al. (1995) A Mycobacterium leprae gene encoding a fibronectin binding protein is used for efficient invasion of epithelial cells and Schwann cells. Infect Immun 63:2652-7
Roach, T I; Barton, C H; Chatterjee, D et al. (1995) Opposing effects of interferon-gamma on iNOS and interleukin-10 expression in lipopolysaccharide- and mycobacterial lipoarabinomannan-stimulated macrophages. Immunology 85:106-13
Oskam, L; Hartskeerl, R A; Hermans, C J et al. (1995) A 46 kDa integral membrane protein from Mycobacterium leprae resembles a number of bacterial and mammalian membrane transport proteins. Microbiology 141 ( Pt 8):1963-8
Pessolani, M C; Smith, D R; Rivoire, B et al. (1994) Purification, characterization, gene sequence, and significance of a bacterioferritin from Mycobacterium leprae. J Exp Med 180:319-27
Brennan, P J; Chatterjee, D; Fujiwara, T et al. (1994) Leprosy-specific neoglycoconjugates: synthesis and application to serodiagnosis of leprosy. Methods Enzymol 242:27-37
Roach, T I; Chatterjee, D; Blackwell, J M (1994) Induction of early-response genes KC and JE by mycobacterial lipoarabinomannans: regulation of KC expression in murine macrophages by Lsh/Ity/Bcg (candidate Nramp). Infect Immun 62:1176-84
Roach, T I; Barton, C H; Chatterjee, D et al. (1993) Macrophage activation: lipoarabinomannan from avirulent and virulent strains of Mycobacterium tuberculosis differentially induces the early genes c-fos, KC, JE, and tumor necrosis factor-alpha. J Immunol 150:1886-96
Daffe, M; McNeil, M; Brennan, P J (1993) Major structural features of the cell wall arabinogalactans of Mycobacterium, Rhodococcus, and Nocardia spp. Carbohydr Res 249:383-98
Hines 2nd, M E; Jaynes, J M; Barker, S A et al. (1993) Isolation and partial characterization of glycolipid fractions from Mycobacterium avium serovar 2 (Mycobacterium paratuberculosis 18) that inhibit activated macrophages. Infect Immun 61:1-7

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