Leukemia Virus Project

Peters, Robert William

Abstract

This contract will support the murine retrovirus genetics and pathogenesis programs of the Viral Oncology and Virology and Cellular Immunology Sections of LIP. The contract effort has 2 main functions: 1) To maintain breeding and small scale production colonies of congenic mouse strains developed for use in the study of host- and virus-related aspects of murine leukemia virus (MuLV) infection. The majority of strains are NFS (inbred NIH Swiss) carrying one or more esotropic murine leukemia virus (MuLV) loci derived from the AKR/N, C58/LN, and C3H/Fg mouse strains. The contract responsibility includes selected typing for expression of MuLV (by preparing tail biopsy extracts and carrying out tissue culture assays by XC plaque testing), setting up mating combinations of appropriate mice at proper intervals, monitoring of health of breeding stock, selective holding for long term observation for disease, shipment of sick animals to LIP and LMM investigators, and provision of specified animals for viral pathogenesis studies. 2) To house and regularly monitor for neoplastic or other disease various strains of mice inoculated with MuLVs. Mice are evaluated for general health, and specifically examined for splenomegaly and lymphadenopathy, respiratory distress, and signs of neurologic disease. There are occasional requirements for XC testing and test bleeding. Sick mice are identified and sent to LIP for autopsy.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Research and Development Contracts (N01)
• Project #: N01AI072622-012
• Application #: 2296690
• Project Start: 1987-02-01
• Project End: 1994-12-31
• Budget Start: 1993-06-30
• Budget End: 1994-12-31
• Fiscal Year: 1993

Institution

• Name: Microbiological Associates, Inc.
• City: Rockville
• State: MD
• Country: United States
• Zip Code: 20850

Publications

Punt, J A; Suzuki, H; Granger, L G et al. (1996) Lineage commitment in the thymus: only the most differentiated (TCRhibcl-2hi) subset of CD4+CD8+ thymocytes has selectively terminated CD4 or CD8 synthesis. J Exp Med 184:2091-9

Doherty, T M; Morse 3rd, H C; Coffman, R L (1995) Modulation of specific T cell responses by concurrent infection with Leishmania major and LP-BM5 murine leukemia viruses. Int Immunol 7:131-8

Tang, Y; Chattopadhyay, S K; Hartley, J W et al. (1994) Clonal outgrowths of T and B cells in SCID mice reconstituted with cells from mice with MAIDS. In Vivo 8:953-9

Justice, M J; Morse 3rd, H C; Jenkins, N A et al. (1994) Identification of Evi-3, a novel common site of retroviral integration in mouse AKXD B-cell lymphomas. J Virol 68:1293-300

Fredrickson, T N; Tang, Y; Chattopadhyay, S K et al. (1993) Retrovirus-induced lymphoproliferation as a model for developing diagnostic criteria for malignant lymphoma in mice. Toxicol Pathol 21:219-28

Tang, Y; Hugin, A W; Hartley, J W et al. (1993) Effects of immunization with the p12 proteins of LP-BM5 defective and ecotropic viruses on development of MAIDS. Arch Virol 129:155-66

Fredrickson, T N; Hartley, J W; Morse 3rd, H C (1993) Early divergence of erythroid lineage suggested by gene rearrangements in mouse hematopoietic neoplasms. Exp Hematol 21:354-7

Makino, M; Sei, Y; Arora, P K et al. (1992) Impaired calcium mobilization in CD4+ and CD8+ T cells in a retrovirus-induced immunodeficiency syndrome, murine AIDS. J Immunol 149:1707-13

Erbe, J G; Green, K A; Crassi, K M et al. (1992) Cytolytic T lymphocytes specific for tumors and infected cells from mice with a retrovirus-induced immunodeficiency syndrome. J Virol 66:3251-6

Cerny, A; Izui, S; Saurat, J H et al. (1992) Epidermal and splenic antigen-presenting cell function in a retrovirally induced murine immunodeficiency syndrome (MAIDS). Arch Dermatol Res 284:189-92