The purpose of this contract is to provide to tuberculosis researchers materials of consistently high quality prepared from M. tuberculosis and to provide a facility to evaluate candidate anti-tuberculosis vaccines in animal models. Mycobacteria are difficult organisms to grow requiring 3-4 weeks to produce a colony on solid culture medium. Because it is transmitted via aerosols, work with the organism will be done in BL3 laboratories. This research materials provided will include irradiated organisms, purified protein, lipid and carbohydrate antigens, culture filtrates, and nucleic acids. The animal models available for consideration include, but are not limited to, the mouse, guinea pig and rabbit. This service will enable and facilitate the participation of researchers (many of whom are without the required BLT facilities needed to culture quantities of M. tuberculosis or evaluate candidate vaccines and drugs under direct challenge conditions) in basic and clinical research projects that require these resources. It also allows the efficient use of facilities and materials as quantities of different materials may be purified from the same source material and standards for comparative evaluation of treatment or prophylactic efficacies developed. Access to research materials, such as purified antigens, prepared under defined protocols, allows greater interchange of information among interested researchers and objective comparison of data from different laboratories using the same materials.

Project Start
1997-07-01
Project End
2004-06-30
Budget Start
2000-09-19
Budget End
2002-03-31
Support Year
Fiscal Year
2000
Total Cost
$839,767
Indirect Cost
Name
Colorado State University-Fort Collins
Department
Microbiology/Immun/Virology
Type
Schools of Veterinary Medicine
DUNS #
112617480
City
Fort Collins
State
CO
Country
United States
Zip Code
80523
Lucas, Megan C; Wolfe, Lisa M; Hazenfield, Rachel M et al. (2015) Fractionation and analysis of mycobacterial proteins. Methods Mol Biol 1285:47-75
Esin, S; Counoupas, C; Aulicino, A et al. (2013) Interaction of Mycobacterium tuberculosis cell wall components with the human natural killer cell receptors NKp44 and Toll-like receptor 2. Scand J Immunol 77:460-9
Cho, Yun Sang; Dobos, Karen M; Prenni, Jessica et al. (2012) Deciphering the proteome of the in vivo diagnostic reagent ""purified protein derivative"" from Mycobacterium tuberculosis. Proteomics 12:979-91
Driessen, Nicole N; Boshoff, Helena I M; Maaskant, Janneke J et al. (2012) Cyanovirin-N inhibits mannose-dependent Mycobacterium-C-type lectin interactions but does not protect against murine tuberculosis. J Immunol 189:3585-92
Laal, Suman (2012) How does Mycobacterium tuberculosis establish infection? J Infect Dis 206:1157-9
West, Nicholas P; Thomson, Scott A; Triccas, James A et al. (2011) Delivery of a multivalent scrambled antigen vaccine induces broad spectrum immunity and protection against tuberculosis. Vaccine 29:7759-65
Tullius, Michael V; Harmston, Christine A; Owens, Cedric P et al. (2011) Discovery and characterization of a unique mycobacterial heme acquisition system. Proc Natl Acad Sci U S A 108:5051-6
Kumar, Parameet; Sen, Manas K; Chauhan, Devendra S et al. (2010) Assessment of the N-PCR assay in diagnosis of pleural tuberculosis: detection of M. tuberculosis in pleural fluid and sputum collected in tandem. PLoS One 5:e10220
McMath, L M; Habel, J E; Sankaran, B et al. (2010) Crystallization and preliminary X-ray crystallographic analysis of a Mycobacterium tuberculosis ferritin homolog, BfrB. Acta Crystallogr Sect F Struct Biol Cryst Commun 66:1657-61
Chim, Nicholas; Iniguez, Angelina; Nguyen, Tran Que et al. (2010) Unusual diheme conformation of the heme-degrading protein from Mycobacterium tuberculosis. J Mol Biol 395:595-608

Showing the most recent 10 out of 117 publications