Abstract

The goal of this project is to investigate the events regulating the proliferation and differentiation of the stem cells required for the reconstitution of hematopoiesis in vivo. The contractor plans to characterize and purify the stem cells that will reconstitute hematopoiesis after marrow transplantation. The influence of various cytokines on this process will also be investigated. A nonhuman primate (baboon) model will be used for this work.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research and Development Contracts (N01)
Project #
N01AI085003-003
Application #
3597251
Study Section
Project Start
1988-09-30
Project End
1993-09-29
Budget Start
1991-05-24
Budget End
1992-09-29
Support Year
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Fred Hutchinson Cancer Research Center
Department
Type
DUNS #
075524595
City
Seattle
State
WA
Country
United States
Zip Code
98109

  Publications

Allen, M D; Weyhrich, J; Gaur, L et al. (1997) Prolonged allogeneic and xenogeneic microchimerism in unmatched primates without immunosuppression by intrathymic implantation of CD34+ donor marrow cells. Cell Immunol 181:127-38
Andrews, R G; Briddell, R A; Knitter, G H et al. (1994) In vivo synergy between recombinant human stem cell factor and recombinant human granulocyte colony-stimulating factor in baboons enhanced circulation of progenitor cells. Blood 84:800-10
Chatta, G S; Andrews, R G; Rodger, E et al. (1993) Hematopoietic progenitors and aging: alterations in granulocytic precursors and responsiveness to recombinant human G-CSF, GM-CSF, and IL-3. J Gerontol 48:M207-12
Andrews, R G; Bensinger, W I; Knitter, G H et al. (1992) The ligand for c-kit, stem cell factor, stimulates the circulation of cells that engraft lethally irradiated baboons. Blood 80:2715-20
Andrews, R G; Bryant, E M; Bartelmez, S H et al. (1992) CD34+ marrow cells, devoid of T and B lymphocytes, reconstitute stable lymphopoiesis and myelopoiesis in lethally irradiated allogeneic baboons. Blood 80:1693-701
Andrews, R G; Bartelmez, S H; Knitter, G H et al. (1992) A c-kit ligand, recombinant human stem cell factor, mediates reversible expansion of multiple CD34+ colony-forming cell types in blood and marrow of baboons. Blood 80:920-7
Andrews, R G; Knitter, G H; Bartelmez, S H et al. (1991) Recombinant human stem cell factor, a c-kit ligand, stimulates hematopoiesis in primates. Blood 78:1975-80