Abstract

The purpose of this contract is to provide 1) a closed, conventional facility for the breeding and maintenance of pedigreed inbred and cogenic mice which are used for plasmacytoma experiments; 2) laboratory facilities for the construction of BALB/Can cogenic strains of mice that carry segments of DBA/2 chromosomes, including the testing of mice using recombinant DNA technology for detecting appropriate marker genes; 3) necessary procedures for inducing plasmacytomas (such as by intraperitoneal injection of pristane, by injection of transforming retroviruses, and the administration of various agents in the drinking water of diet that inhibits plasmacytomagenesis (e.g. indomethacin); 4) a facility for maintaining and observing mice and detecting placytomas in mice during the time when tumors develop; 5) a facility for transplanting, preserving by cryopreservation, and recovering of tumors for purposes of shipping the tumors to other laboratories; 6) a facility for storing frozen tumor tissues and other genomic experiments; and 7) a facility for the maintenance of wild mice.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Biology And Diagnosis (NCI)
Type
Research and Development Contracts (N01)
Project #
N01CB021075-000
Application #
3701903
Study Section
Project Start
1992-12-18
Project End
1997-01-31
Budget Start
1992-02-01
Budget End
1993-01-31
Support Year
Fiscal Year
1992
Total Cost
Indirect Cost

  Institution

Name
Covance Laboratories, Inc.
Department
Type
DUNS #
City
Vienna
State
VA
Country
United States
Zip Code
22182

  Publications

Mock, B A; Connelly, M A; McBride, O W et al. (1996) Plasmacytoma-associated neuronal glycoprotein, Pang, maps to mouse chromosome 6 and human chromosome 3. Genomics 34:226-8
Mock, B A; Connelly, M A; McBride, O W et al. (1995) CHUK, a conserved helix-loop-helix ubiquitous kinase, maps to human chromosome 10 and mouse chromosome 19. Genomics 27:348-51
Mock, B A; Padlan, C; Kozak, C A et al. (1994) The gene for mouse p58cdc2L1 (Cdc2l1) protein kinase maps to distal mouse chromosome 4. Mamm Genome 5:191-2
Dosik, J K; Barton, C H; Holiday, D L et al. (1994) An Nramp-related sequence maps to mouse chromosome 17. Mamm Genome 5:458-60
Vogel, S N; Wax, J S; Perera, P Y et al. (1994) Construction of a BALB/c congenic mouse, C.C3H-Lpsd, that expresses the Lpsd allele: analysis of chromosome 4 markers surrounding the Lps gene. Infect Immun 62:4454-9
Mock, B A; Krall, M M; Dosik, J K (1993) Genetic mapping of tumor susceptibility genes involved in mouse plasmacytomagenesis. Proc Natl Acad Sci U S A 90:9499-503
Cerretti, D P; Nelson, N; Kozlosky, C J et al. (1993) The murine homologue of the human interleukin-8 receptor type B maps near the Ity-Lsh-Bcg disease resistance locus. Genomics 18:410-3
Krall, M; Ruff, N; Zimmerman, K et al. (1992) Isolation and mapping of four new DNA markers from mouse chromosome 4. Mamm Genome 3:653-5