Abstract

The efficacy of a variety of chemopreventive agents to reduce or inhibit colon cancer using male Fisher 344 rats treated with a carcinogen +- chemopreventive agents is being assessed. Aberrant crypt formation four weeks after the first carcinogen exposure is being measured. All rats, approximately 8 weeks old, are treated with two injections of 15 mg of azoxymethane (AOM) per kg of body weight one week apart. An dose selection study is performed prior to the chemoprevention study for each agent at five doses. For protocol A: Administer each agent at five doses to approximately seven-week-old rats. Feed the rats the test chemopreventive agent from one week prior to and four weeks following the first carcinogen treatment. Then kill the rats and score the aberrant crypts. For Protocol B: Administration of the chemopreventive agent shall begin 4 weeks after the first carcinogen dose and continued for four weeks. At eight weeks following the first carcinogen treatment aberrant crypts will be scored. For both protocols, the number of aberrant crypt foci per colon and the number of aberrant crypts per foci from unsectioned methylene-blue stained colons are being determined. The two doses to be used in the aberrant crypt foci study are determined from data produced in the rangefinding assay and obtain approval from the Project Officer. Ten animals are being used per group. There are 20 agents in protocol A and 10 in protocol B.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Prevention And Control (NCI)
Type
Research and Development Contracts (N01)
Project #
N01CN055175-000
Application #
2302678
Study Section
Project Start
1995-09-30
Project End
Budget Start
1995-09-30
Budget End
1997-09-29
Support Year
Fiscal Year
1995
Total Cost
Indirect Cost

  Institution

Name
University of Toledo
Department
Pathology
Type
Schools of Medicine
DUNS #
807418939
City
Toledo
State
OH
Country
United States
Zip Code
43614