Abstract

The investigators will isolate organ-specific epithelial and fibroblast cells and lymphocyte cells from phenotypically normal patients with 6 dominantly heritable cancer syndromes. Similar control cultures will be established from normal patients without the inherited mutation(s). Cells cultures will be used to characterize the cytotoxicity and mRNA expression patterns induced by in vitro cell exposure. Biopsy samples obtained from phenotypically normal, medicinally (chemopreventive) treated and nontreated patients and controls will be used to evaluate differentially displayed mRNA patterns. The in vitro and in vivo mRNA expression profiles will be compared to characterize the effects of chemopreventive agents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Prevention And Control (NCI)
Type
Research and Development Contracts (N01)
Project #
N01CN095037-001
Application #
6345588
Study Section
Project Start
1999-09-30
Project End
2002-03-29
Budget Start
2000-08-21
Budget End
2001-09-29
Support Year
Fiscal Year
2000
Total Cost
$975,469
Indirect Cost

  Institution

Name
Fox Chase Cancer Center
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19111

  Publications

Peri, Suraj; Devarajan, Karthik; Yang, Dong-Hua et al. (2013) Meta-analysis identifies NF-?B as a therapeutic target in renal cancer. PLoS One 8:e76746
Bellacosa, Alfonso; Godwin, Andrew K; Peri, Suraj et al. (2010) Altered gene expression in morphologically normal epithelial cells from heterozygous carriers of BRCA1 or BRCA2 mutations. Cancer Prev Res (Phila) 3:48-61
Caretti, Elena; Devarajan, Karthik; Coudry, Renata et al. (2008) Comparison of RNA amplification methods and chip platforms for microarray analysis of samples processed by laser capture microdissection. J Cell Biochem 103:556-63
Upson, John J; Stoyanova, Radka; Cooper, Harry S et al. (2004) Optimized procedures for microarray analysis of histological specimens processed by laser capture microdissection. J Cell Physiol 201:366-73
Stoyanova, Radka; Upson, John J; Patriotis, Christos et al. (2004) Use of RNA amplification in the optimal characterization of global gene expression using cDNA microarrays. J Cell Physiol 201:359-65
Stoyanova, Radka; Clapper, Margie L; Bellacosa, Alfonso et al. (2004) Altered gene expression in phenotypically normal renal cells from carriers of tumor suppressor gene mutations. Cancer Biol Ther 3:1313-21
Cutler, N Shane; Graves-Deal, Ramona; LaFleur, Bonnie J et al. (2003) Stromal production of prostacyclin confers an antiapoptotic effect to colonic epithelial cells. Cancer Res 63:1748-51
Manduchi, E; Grant, G R; McKenzie, S E et al. (2000) Generation of patterns from gene expression data by assigning confidence to differentially expressed genes. Bioinformatics 16:685-98