This laboratory will be sent fresh solid tumor specimens which they will then disaggregate and incubate under appropriate growth conditions. The laboratory personnel will then make slides from mitotically active cultures. When metaphases have been identified under the microscope, the chromosomes in them will be stained by suitable methods (primarily with Wright's stain, giemsatrypsin or quinacrine) and the individual cells will be analyzed to determine the karyotype. Photographs will be taken of good metaphase spreads, and the chromosomes in them will be studied in detail. The goal of the contract is to identify changes in chromosome number and/or structure that may be important in the etiology of specific types of solid tumors.

Agency
National Institute of Health (NIH)
Institute
Division of Cancer Epidemiology And Genetics (NCI)
Type
Research and Development Contracts (N01)
Project #
N01CP071018-003
Application #
3633025
Study Section
Project Start
1987-06-30
Project End
1992-06-29
Budget Start
1989-03-20
Budget End
1990-06-29
Support Year
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Roswell Park Cancer Institute Corp
Department
Type
DUNS #
City
Buffalo
State
NY
Country
United States
Zip Code
14263
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