National Transgenic Mouse Facility

Swanson, Mark

Abstract

The NICHD will provide, through the contract mechanism, a facility for supplying transgenic mice to the scientific community. The demand for transgenic mice is substantial, but the ability to produce them is limited. Thus, the aim of this contract is to make available to the general scientific community at large access to the technology necessary for the production of transgenic mice. More specifically, the Contractor shall be responsible for the following: a. accepting donor DNA probes either as linear DNA, or in a cloning vector but not as bacteria containing plasmid. b. confirming the identity of the donor DNA. c. generating by microinjection techniques transgenic mice using the DNA insert provided by the donor. d. confirming integration of donor DNA into the genome of the mice produced. e. maintaining the transgenic mice at the facility and distributing the mice to individuals in the scientific community who have requested the service. f. instituting a program to publicize the availability of this service.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
• Type: Research and Development Contracts (N01)
• Project #: N01HD002911-004
• Application #: 2314476
• Project Start: 1990-04-01
• Project End: 1993-09-30
• Budget Start: 1993-01-26
• Budget End: 1993-12-31
• Fiscal Year: 1993

Institution

• Name: Dnx, Inc.
• City: Athens
• State: OH
• Country: United States
• Zip Code: 45701

Publications

Sunday, M E; Haley, K J; Sikorski, K et al. (1999) Calcitonin driven v-Ha-ras induces multilineage pulmonary epithelial hyperplasias and neoplasms. Oncogene 18:4336-47

vanWert, J M; Panek, H R; Wolfe, S A et al. (1998) The TE promoter element of the histone H1t gene is essential for transcription in transgenic mouse primary spermatocytes. Biol Reprod 59:704-10

Rhodes, K; Oshima, R G (1998) A regulatory element of the human keratin 18 gene with AP-1-dependent promoter activity. J Biol Chem 273:26534-42

Johnston, D; Hatzis, D; Sunday, M E (1998) Expression of v-Ha-ras driven by the calcitonin/calcitonin gene-related peptide promoter: a novel transgenic murine model for medullary thyroid carcinoma. Oncogene 16:167-77

Neznanov, N; Umezawa, A; Oshima, R G (1997) A regulatory element within a coding exon modulates keratin 18 gene expression in transgenic mice. J Biol Chem 272:27549-57

Grimes Jr, S R; van Wert, J; Wolfe, S A (1997) Regulation of transcription of the testis-specific histone H1t gene by multiple promoter elements. Mol Biol Rep 24:175-84

Erickson, R P; Kessler, S; Kremling, H et al. (1996) Species variation in the testicular angiotensin converting enzyme promoter studied in transgenic mice. Mol Reprod Dev 44:324-31

Joseph, R M; Li, T (1996) Overexpression of Bcl-2 or Bcl-XL transgenes and photoreceptor degeneration. Invest Ophthalmol Vis Sci 37:2434-46

Robles, A I; Larcher, F; Whalin, R B et al. (1996) Expression of cyclin D1 in epithelial tissues of transgenic mice results in epidermal hyperproliferation and severe thymic hyperplasia. Proc Natl Acad Sci U S A 93:7634-8

Nakayama, M; Stauffer, J; Cheng, J et al. (1996) Common core sequences are found in skeletal muscle slow- and fast-fiber-type-specific regulatory elements. Mol Cell Biol 16:2408-17