Human milk is a unique biological fluid that has functional properties that appear to be as important if not more so than its nutritional properties. The overall goal of the project is to: 1) improve methods of isolating, characterizing and analyzing fat globules of human colostrum and milk; 2) develop a method of preparing milk and colostrum for microscopy such that the complete material can be viewed by light and electron microscopy; 3) using the above procedures and other available methods to enumerate and quantify the cytoplasmic cresents associated with fat globules in milk and colostrum; 4) evaluate factors which may influence their formation and devise a procedure for isolating the crescent material from them; 5) perfect a technique for isolating and purifying human fat globules based on centrifuging them from the milk or colostrum (up) through an overlying water or buffer layer; 6) employing the procedure in 4, develop or apply suitable methods for determining protein, phospholipid and cholesterol per gram of globules; and 7) isolate fat globules from colostrum and determine the pattern and concentration of carotenoids they contain.

Project Start
1986-02-01
Project End
1989-01-31
Budget Start
1986-06-29
Budget End
1987-01-31
Support Year
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Type
Schools of Medicine
DUNS #
077758407
City
La Jolla
State
CA
Country
United States
Zip Code
92093
Patton, S (2001) MUC1 and MUC-X, epithelial mucins of breast and milk. Adv Exp Med Biol 501:35-45
Patton, S (1994) Detection of large fragments of the human milk mucin MUC-1 in feces of breast-fed infants. J Pediatr Gastroenterol Nutr 18:225-30
Patton, S; Muller, L D (1992) Genetic polymorphism of the epithelial mucin, PAS-I, in milk samples from the major dairy breeds. J Dairy Sci 75:863-7
Campana, W M; Josephson, R V; Patton, S (1992) Presence and genetic polymorphism of an epithelial mucin in milk of the goat (Capra hircus). Comp Biochem Physiol B 103:261-6
Patton, S; Patton, R S (1990) Genetic polymorphism of PAS-I, the mucin-like glycoprotein of bovine milk fat globule membrane. J Dairy Sci 73:3567-74
Patton, S; Canfield, L M; Huston, G E et al. (1990) Carotenoids of human colostrum. Lipids 25:159-65
Huston, G E; Patton, S (1990) Factors related to the formation of cytoplasmic crescents on milk fat globules. J Dairy Sci 73:2061-6
Patton, S; Huston, G E; Jenness, R et al. (1989) Differences between individuals in high-molecular weight glycoproteins from mammary epithelia of several species. Biochim Biophys Acta 980:333-8
Buchheim, W; Welsch, U; Huston, G E et al. (1988) Glycoprotein filament removal from human milk fat globules by heat treatment. Pediatrics 81:141-6
Patton, S; Huston, G E (1988) Incidence and characteristics of cell pieces on human milk fat globules. Biochim Biophys Acta 965:146-53

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