Biological Testing Facility

Reel, Jerry R.

Abstract

The objective of this contract is to continue the operation and maintenance of a Biological Testing Facility for the exclusive use of the Contraceptive Development Branch. Included in these objectives are broad spectrum and discrete antifertility and biochemical tests, screens and assays for classical hormonal activity radioreceptor and radioimmunoassays of both natural hormones and new potential contraceptive steroids, preclinical pharmacokinetic and toxicology studies and mechanism of action studies in rodents and rabbits. Some of these studies will be undertaken under GLP guidelines and reports submitted to the FDA in support of INDs. Rodents, rabbits and a colony of rhesus monkeys will be maintained under the contract. The facility will be AAALAC accredited.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
• Type: Research and Development Contracts (N01)
• Project #: N01HD063259-009
• Application #: 6344573
• Project Start: 1996-09-01
• Project End: 2001-06-30
• Budget Start: 2000-07-01
• Budget End: 2001-01-31
• Fiscal Year: 2000
• Total Cost: $1,872,664

Institution

• Name: Bioqual, Inc.
• City: Rockville
• State: MD
• Country: United States
• Zip Code: 20850

Publications

Tash, Joseph S; Chakrasali, Ramappa; Jakkaraj, Sudhakar R et al. (2008) Gamendazole, an orally active indazole carboxylic acid male contraceptive agent, targets HSP90AB1 (HSP90BETA) and EEF1A1 (eEF1A), and stimulates Il1a transcription in rat Sertoli cells. Biol Reprod 78:1139-52

Attardi, Barbara J; Burgenson, Janet; Hild, Sheri A et al. (2004) In vitro antiprogestational/antiglucocorticoid activity and progestin and glucocorticoid receptor binding of the putative metabolites and synthetic derivatives of CDB-2914, CDB-4124, and mifepristone. J Steroid Biochem Mol Biol 88:277-88

Hild, Sheri Ann; Attardi, Barbara J; Reel, Jerry R (2004) The ability of a gonadotropin-releasing hormone antagonist, acyline, to prevent irreversible infertility induced by the indenopyridine, CDB-4022, in adult male rats: the role of testosterone. Biol Reprod 71:348-58

Attardi, Barbara J; Burgenson, Janet; Hild, Sheri A et al. (2002) CDB-4124 and its putative monodemethylated metabolite, CDB-4453, are potent antiprogestins with reduced antiglucocorticoid activity: in vitro comparison to mifepristone and CDB-2914. Mol Cell Endocrinol 188:111-23

Hild, S A; Meistrich, M L; Blye, R P et al. (2001) Lupron depot prevention of antispermatogenic/antifertility activity of the indenopyridine, CDB-4022, in the rat. Biol Reprod 65:165-72

Hild, S A; Reel, J R; Hoffman, L H et al. (2000) CDB-2914: anti-progestational/anti-glucocorticoid profile and post-coital anti-fertility activity in rats and rabbits. Hum Reprod 15:822-9

Larner, J M; Reel, J R; Blye, R P (2000) Circulating concentrations of the antiprogestins CDB-2914 and mifepristone in the female rhesus monkey following various routes of administration. Hum Reprod 15:1100-6

Reel, J R; Hild-Petito, S; Blye, R P (1998) Antiovulatory and postcoital antifertility activity of the antiprogestin CDB-2914 when administered as single, multiple, or continuous doses to rats. Contraception 58:129-36

Hild-Petito, S; Veazey, R S; Larner, J M et al. (1998) Effects of two progestin-only contraceptives, Depo-Provera and Norplant-II, on the vaginal epithelium of rhesus monkeys. AIDS Res Hum Retroviruses 14 Suppl 1:S125-30