Abstract

The purpose of this project is to develop a safe, relatively inexpensive, accurate and non-invasive technique for the prenatal diagnosis of genetic disorders by isolating fetal cells in maternal peripheral circulation during the first trimester of pregnancy. Separation will be accomplished by flow cytometry with trophoblast antibodies and HLA antibodies. Methods will be developed for culturing fetal cells. Fetal aneuploidy and polyploidy will be detected by measuring fetal cellular DNA with flow cytometry (DNA indexing), a sensitive method developed by the investigators. These techniques can be used for the prenatal diagnosis of cytogenetic, biochemical or DNA abnormalities.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research and Development Contracts (N01)
Project #
N01HD082904-002
Application #
2316314
Study Section
Project Start
1987-12-31
Project End
1989-12-30
Budget Start
1988-09-08
Budget End
1988-12-30
Support Year
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Tennessee Health Science Center
Department
Obstetrics & Gynecology
Type
Schools of Medicine
DUNS #
941884009
City
Memphis
State
TN
Country
United States
Zip Code
38163

  Publications

Shulman, L P; Moretti, M L; Tharapel, A T et al. (1993) Mode of ascertainment is critical in assessing safety of percutaneous umbilical blood sampling. Am J Perinatol 10:27-9
Phillips, O P; Simpson, J L; Morgan, C D et al. (1992) Unexplained elevated maternal serum alpha-fetoprotein is not predictive of adverse perinatal outcome in an indigent urban population. Am J Obstet Gynecol 166:978-82
Elias, S; Annas, G J; Simpson, J L (1991) Carrier screening for cystic fibrosis: implications for obstetric and gynecologic practice. Am J Obstet Gynecol 164:1077-83
Shulman, L P; Meyers, C M; Simpson, J L et al. (1990) Fetomaternal transfusion depends on amount of chorionic villi aspirated but not on method of chorionic villus sampling. Am J Obstet Gynecol 162:1185-8
Tipton, R E; Tharapel, A T; Chang, H H et al. (1989) Rapid chromosome analysis with the use of spontaneously dividing cells derived from umbilical cord blood (fetal and neonatal). Am J Obstet Gynecol 161:1546-8
Elias, S; Simpson, J L; Shulman, L P et al. (1989) Transabdominal chorionic villus sampling for first-trimester prenatal diagnosis. Am J Obstet Gynecol 160:879-84;discussion 884-6
Elias, S; Chandler, R W; Wachtel, S (1988) Relative deoxyribonucleic acid content of interphase leukocytes by flow cytometry: a method for indirect diagnosis of chromosomal abnormalities with potential for prenatal diagnosis. Am J Obstet Gynecol 158:808-18