Recombinant Fsh and Lh Antagonists

Boime, Irving

Abstract

The objectives of this project are to synthesize and test recombinant FSH and LH analogs including the deglycosylated forms as potential male contraceptive agents. The prime contractor will focus on designing FSH antagonists by altering protein and carbohydrate structures and redesigning new vectors from the existing vector by inclusion of amplifiable genes and multiple promotors (LTR) using recombinant DNA technique. The subcontractor will carry out the large-scale production of wild-type FSH, LH and deglycosylated FSH and LH by means of a bioreactor.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
• Type: Research and Development Contracts (N01)
• Project #: N01HD092922-000
• Application #: 3654104
• Project Start: 1989-09-30
• Project End: 1992-09-29
• Budget Start: 1989-09-30
• Budget End: 1990-09-29
• Fiscal Year: 1989

Institution

• Name: Washington University
• Type: Schools of Medicine
• DUNS #: 062761671
• City: Saint Louis
• State: MO
• Country: United States
• Zip Code: 63130

Publications

Garcia-Campayo, V; Sato, A; Hirsch, B et al. (1997) Design of stable biologically active recombinant lutropin analogs. Nat Biotechnol 15:663-7

Sugahara, T; Sato, A; Kudo, M et al. (1996) Expression of biologically active fusion genes encoding the common alpha subunit and the follicle-stimulating hormone beta subunit. Role of a linker sequence. J Biol Chem 271:10445-8

Sugahara, T; Pixley, M R; Fares, F et al. (1996) Characterization of the O-glycosylation sites in the chorionic gonadotropin beta subunit in vivo using site-directed mutagenesis and gene transfer. J Biol Chem 271:20797-804

Sugahara, T; Grootenhuis, P D; Sato, A et al. (1996) Expression of biologically active fusion genes encoding the common alpha subunit and either the CG beta or FSH beta subunits: role of a linker sequence. Mol Cell Endocrinol 125:71-7

Sugahara, T; Pixley, M R; Minami, S et al. (1995) Biosynthesis of a biologically active single peptide chain containing the human common alpha and chorionic gonadotropin beta subunits in tandem. Proc Natl Acad Sci U S A 92:2041-5