The goal of this contract is to develop novel mass spectrometric methodologies and technology for the quantitative analysis of the entire proteome from samples as small as individual cells. A comprehensive approach to sample generation, technology development, and data analysis will generate not only new instrumentation but also important physiological data that will be rapidly and freely disseminated. The work will be carried out in 5 separate components with the following goals and objectives. I.) Proteomics and Technology Development: Development of novel mass spectrometric methodologies and technology for the quantitative analysis of the entire proteome of a single cell. II.) Animal Models and Experimental Systems: The Animal Models and Experimental Systems Component, is charged with the development, maintenance and production of the animal and cell culture models of angiogenesis to be studied. III.) Protein analysis and Technology Implementation: To implement the technological advances in analytical mass spectrometry (component I) by merging them with existing analytical capabilities to identify and quantify the proteome of individual cells or tissue samples and cell lines during angiogenesis. IV.) Bioinformatics: To apply state-of-the-art information science techniques to applications in proteomics. V.) Administration: To integrate and facilitate the activities of the scientific components.
