Dr. Kenneth Williams NHLBI-HL-02-04Yale University We propose to define changes in the relative levels of protein and phosphoprotein expression that underlie, define, and result from pathophysiologies related to: hematopoiesis, vascular biology, and hypertension. To assist with analyzing the enormous amounts of protein expression data that will be produced and to archive this data so it can be analyzed, queried, and disseminated via web interface, we will design and institute the NHLBI/Yale Expression Database. This database will be linked and cross-correlated with mRNA expression data in the Yale Microarray Database and with functional and other bioinformatics databases. To take full advantage of the ability of protein profiling to uncover proteins that play key roles in pathophysiologies of interest will require that equally effective biotechnologies be developed for modulating the in vivo activities of these protein targets. Hence, we propose also a tightly coordinated program for improving nd utilizing cell permeable synthetic peptide delivery systems to block specific protein:protein interactions - with the goal of increasing our understanding of the disease process which, in turn, should lead to more effective treatments for these diseases
