Abstract

application): The overall goal of this program project is to develop a vaccine for coccidioidomycosis. Immunization experiments require the production of recombinant protein. In addition, the investigators propose to use DNA immunization as an alternative to immunization with protein. The purpose of this core is to produce recombinant protein in sufficient quantities to allow Projects I, II, III, and V to conduct their proposed research. In addition, plasmid DNA will be used by Project V for genetic immunization. The core will streamline and centralize production of these essential reagents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI037232-08
Application #
6657472
Study Section
Special Emphasis Panel (ZAI1)
Project Start
2002-09-01
Project End
2003-07-31
Budget Start
Budget End
Support Year
8
Fiscal Year
2002
Total Cost
$157,141
Indirect Cost

  Institution

Name
Veterans Medical Research Fdn/San Diego
Department
Type
DUNS #
933863508
City
San Diego
State
CA
Country
United States
Zip Code
92161

  Publications

Hung, Chiung-Yu; Xue, Jianmin; Cole, Garry T (2007) Virulence mechanisms of coccidioides. Ann N Y Acad Sci 1111:225-35
Nosanchuk, Joshua D; Yu, Jieh-Juen; Hung, Chiung-Yu et al. (2007) Coccidioides posadasii produces melanin in vitro and during infection. Fungal Genet Biol 44:517-20
Shubitz, Lisa F; Yu, Jieh-Juen; Hung, Chiung-Yu et al. (2006) Improved protection of mice against lethal respiratory infection with Coccidioides posadasii using two recombinant antigens expressed as a single protein. Vaccine 24:5904-11
Mirbod-Donovan, Fariba; Schaller, Ruth; Hung, Chiung-Yu et al. (2006) Urease produced by Coccidioides posadasii contributes to the virulence of this respiratory pathogen. Infect Immun 74:504-15
Xue, Jianmin; Hung, Chiung-Yu; Yu, Jieh-Juen et al. (2005) Immune response of vaccinated and non-vaccinated mice to Coccidioides posadasii infection. Vaccine 23:3535-44
Hung, Chiung-Yu; Seshan, Kalpathi R; Yu, Jieh-Juen et al. (2005) A metalloproteinase of Coccidioides posadasii contributes to evasion of host detection. Infect Immun 73:6689-703
Johannesson, Hanna; Townsend, Jeffrey P; Hung, Chiung-Yu et al. (2005) Concerted evolution in the repeats of an immunomodulating cell surface protein, SOWgp, of the human pathogenic fungi Coccidioides immitis and C. posadasii. Genetics 171:109-17
Cole, G T; Xue, J M; Okeke, C N et al. (2004) A vaccine against coccidioidomycosis is justified and attainable. Med Mycol 42:189-216
Johannesson, Hanna; Vidal, Pilar; Guarro, Josep et al. (2004) Positive directional selection in the proline-rich antigen (PRA) gene among the human pathogenic fungi Coccidioides immitis, C. posadasii and their closest relatives. Mol Biol Evol 21:1134-45
Delgado, Nelson; Xue, Jianmin; Yu, Jieh-Juen et al. (2003) A recombinant beta-1,3-glucanosyltransferase homolog of Coccidioides posadasii protects mice against coccidioidomycosis. Infect Immun 71:3010-9

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