Abstract

This core will supply large scale amount of eukaryotic and prokaryotic expression system-produced proteins. Specifically, CD4/1-183 and gp41 will be made in T7-based E. coli expression systems as inclusion bodies. In addition, Lec3.2.8.1 generated ADA and SF2 HIV-gp120 and gp160 proteins will be produced. Large scale mAb production will also be the responsibility of this core. As required, selenomethionine labeling of eukaryotically expressed protein will be undertaken for x-ray crystallography as well as /15N labeling of E. coli produced proteins for NMR analysis. Large scale mini-protein will be performed by this core once appropriate constructs have been generated. The assay component of the core will test small molecules identified by SAR by NMR as binders to CD4, gp120 or gp41 for their functional effects on CD4-gp120 interaction, HIV viral infectivity and for class II MHC interaction using a set of nine assays as detailed in the following section.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program Projects (P01)
Project #
5P01AI043649-03
Application #
6336265
Study Section
Project Start
2000-08-01
Project End
2001-07-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
3
Fiscal Year
2000
Total Cost
$171,998
Indirect Cost

  Institution

Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02215

  Publications

Tomaras, Georgia D; Montefiori, David C (2014) Spectrum of HIV antibodies in vaccine and disease. Curr Opin HIV AIDS 9:207-9
Hatfield, Stephen; Belikoff, Bryan; Lukashev, Dmitriy et al. (2009) The antihypoxia-adenosinergic pathogenesis as a result of collateral damage by overactive immune cells. J Leukoc Biol 86:545-8
Song, Likai; Sun, Zhen-Yu J; Coleman, Kate E et al. (2009) Broadly neutralizing anti-HIV-1 antibodies disrupt a hinge-related function of gp41 at the membrane interface. Proc Natl Acad Sci U S A 106:9057-62
Sun, Zhen-Yu J; Oh, Kyoung Joon; Kim, Mikyung et al. (2008) HIV-1 broadly neutralizing antibody extracts its epitope from a kinked gp41 ectodomain region on the viral membrane. Immunity 28:52-63
Kim, Mikyung; Qiao, Zhisong; Yu, Jessica et al. (2007) Immunogenicity of recombinant human immunodeficiency virus type 1-like particles expressing gp41 derivatives in a pre-fusion state. Vaccine 25:5102-14
Kim, Mikyung; Qiao, Zhi-Song; Montefiori, David C et al. (2005) Comparison of HIV Type 1 ADA gp120 monomers versus gp140 trimers as immunogens for the induction of neutralizing antibodies. AIDS Res Hum Retroviruses 21:58-67
Reche, Pedro A; Zhang, Hong; Glutting, John-Paul et al. (2005) EPIMHC: a curated database of MHC-binding peptides for customized computational vaccinology. Bioinformatics 21:2140-1
Qiao, Zhi-Song; Kim, Mikyung; Reinhold, Bruce et al. (2005) Design, expression, and immunogenicity of a soluble HIV trimeric envelope fragment adopting a prefusion gp41 configuration. J Biol Chem 280:23138-46
Chen, Bing; Vogan, Erik M; Gong, Haiyun et al. (2005) Determining the structure of an unliganded and fully glycosylated SIV gp120 envelope glycoprotein. Structure 13:197-211
Chen, Bing; Cheng, Yifan; Calder, Lesley et al. (2004) A chimeric protein of simian immunodeficiency virus envelope glycoprotein gp140 and Escherichia coli aspartate transcarbamoylase. J Virol 78:4508-16

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