The Microscopy Core and Flow Cytometry (Core B) will provide expertise and service related to standard electron microscopy (EM), as well as fluorescent microscopy and flow cytometry as needed by each of the three project components. Such a centralized facility for specimen preparation and imaging will be cost- effective and facilitate more orderly and efficient use of the EM and fluorescent microscope resources to complete the proposed projects in a timely fashion. The requested budget is modest with a 50% salary for a microscopy/imaging lab manager and as well as a research technician and 50% service contract for the EM and fluorescent microscopes. A new addition is 24% time for a flow cytometry expert. The specific functions of the core will include: (1) specimen preparation for electron microscopy; (2) imaging of viruses, recombinant particles, and their complexes with ?carbohydrate and antibodies, reconstituted lipid vesicles to evaluate purity and concentration; (3) imaging of fecal samples from calicivirus infected animals and humans; (4) imaging of stained thin sections of virus infected cells; (5) immuno-electron microscopy; (6) data acquisition, sharing, and archiving; (7) scheduling and day-to-day EM maintenance; (8) specimen preparation for fluorescent microscopy; (9) flow cytometry to obtain quantitative data on virus-infected cells and on PBMCs collected from norovirus-infected individuals (10) scheduling and day-to-day maintenance of the microscopes and flow cytometers. The Core Directors and Associate Director will coordinate all aspects of the Microscopy and Flow Cytometry Core.
| Alvarado, Gabriela; Ettayebi, Khalil; Atmar, Robert L et al. (2018) Human Monoclonal Antibodies That Neutralize Pandemic GII.4 Noroviruses. Gastroenterology 155:1898-1907 |
| Costantini, Veronica; Morantz, Esther K; Browne, Hannah et al. (2018) Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation. Emerg Infect Dis 24:1453-1464 |
| Bányai, Krisztián; Estes, Mary K; Martella, Vito et al. (2018) Viral gastroenteritis. Lancet 392:175-186 |
| Cortes-Penfield, Nicolas W; Ramani, Sasirekha; Estes, Mary K et al. (2017) Prospects and Challenges in the Development of a Norovirus Vaccine. Clin Ther 39:1537-1549 |
| Ramani, Sasirekha; Neill, Frederick H; Ferreira, Jennifer et al. (2017) B-Cell Responses to Intramuscular Administration of a Bivalent Virus-Like Particle Human Norovirus Vaccine. Clin Vaccine Immunol 24: |
| Hurwitz, Amy M; Huang, Wanzhi; Estes, Mary K et al. (2017) Deep sequencing of phage-displayed peptide libraries reveals sequence motif that detects norovirus. Protein Eng Des Sel 30:129-139 |
| Sharma, Sumit; Carlsson, Beatrice; Czakó, Rita et al. (2017) Human Sera Collected between 1979 and 2010 Possess Blocking-Antibody Titers to Pandemic GII.4 Noroviruses Isolated over Three Decades. J Virol 91: |
| Shanker, Sreejesh; Hu, Liya; Ramani, Sasirekha et al. (2017) Structural features of glycan recognition among viral pathogens. Curr Opin Struct Biol 44:211-218 |
| Zou, Winnie Y; Blutt, Sarah E; Crawford, Sue E et al. (2017) Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections. Methods Mol Biol : |
| Yu, Huimin; Hasan, Nesrin M; In, Julie G et al. (2017) The Contributions of Human Mini-Intestines to the Study of Intestinal Physiology and Pathophysiology. Annu Rev Physiol 79:291-312 |
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