Abstract

This is a renewal grant application for an ongoing program project whose overall objective is the study of the chemistry and metabolism of connective tissues. The program is composed of five projects and two core units. Project 1 will deal with the genetic regulation of elastin biosynthesis and metabolism. Project 2 will explore the mechanisms of post-translational glycosylation of different collagens and the properties and formation of matrix glycoproteins. Project 3 will continue to study the biochemistry and immunology of basement membranes and will extend this work into a study of genetic regulation. Project 4 will investigate the properties of basement membrane proteins involved in the pathogenesis of interstitial nephritis. Project 5 will extend the knowledge gained from the study of connective tissue components into the properties of cells generating these proteins and in comparing properties of cells from different tissues during differentiation. An administrative core and a core analytical facility will supplement the research efforts.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases (NIADDK)
Type
Research Program Projects (P01)
Project #
5P01AM020553-09
Application #
3092259
Study Section
(SRC)
Project Start
1978-05-01
Project End
1988-04-30
Budget Start
1985-05-01
Budget End
1986-04-30
Support Year
9
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University City Science Center
Department
Type
DUNS #
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Howard, P S; Myers, J C; Gorfien, S F et al. (1991) Progressive modulation of endothelial phenotype during in vitro blood vessel formation. Dev Biol 146:325-38
Boye, E; Vetrie, D; Flinter, F et al. (1991) Major rearrangements in the alpha 5(IV) collagen gene in three patients with Alport syndrome. Genomics 11:1125-32
Myers, J C; Howard, P S; Walther, S E et al. (1990) Collagen and fibronectin gene expression in cultured endothelial cells. Ann N Y Acad Sci 580:120-31
Myers, J C; Jones, T A; Pohjolainen, E R et al. (1990) Molecular cloning of alpha 5(IV) collagen and assignment of the gene to the region of the X chromosome containing the Alport syndrome locus. Am J Hum Genet 46:1024-33
Pihlajaniemi, T; Pohjolainen, E R; Myers, J C (1990) Complete primary structure of the triple-helical region and the carboxyl-terminal domain of a new type IV collagen chain, alpha 5(IV). J Biol Chem 265:13758-66
Howard, P S; Macarak, E J (1989) Localization of collagen types in regional segments of the fetal bovine aorta. Lab Invest 61:548-55
Pihlajaniemi, T; McKeon, J; Gay, S et al. (1989) Increased expression of the gene for the pro alpha 1(IV) chain of basement-membrane procollagen in cultured skin fibroblasts from two variants of osteogenesis imperfecta. Biochem J 257:439-45
Macarak, E J; Gorfien, S; MacGregor, R R (1989) Modulation of endothelial fibronectin synthesis by polymorphonuclear granulocytes. J Cell Physiol 139:517-23
Frankel, F R; Hsu, C Y; Myers, J C et al. (1988) Regulation of alpha 2(I), alpha 1(III), and alpha 2(V) collagen mRNAs by estradiol in the immature rat uterus. DNA 7:347-54
Alper, R (1988) The bovine corneal SGP-complex is related to the tissue form of type VI collagen. Curr Eye Res 7:31-42

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