Core C2. Dynamic Light Scattering (DLS) and Atomic Force Microscopy (AFM) Paul A. Janmey, Ph.D.All four projects in this program will be supported by the atomic force microscopy (AFM) and dynamic light scattering(DLS) core which will provide structural and in some cases mechanical data from measurements of hydrated samples ofmotor molecules either alone or bound to filaments (F-actin or microtubules). Specialized facilities at the Institute forMedicine and Engineering and the Laboratory for Research in the Structure of Matter at Penn will provide novelquantitative methods to measure the conformations of proteins and protein complexes in solution (DLS) and to imagethese complexes alone or bound to cytoskeletal filaments (AFM). Studies of hydrated samples obtained by AFM willbe integrated with the higher resolution images of dehydrated samples obtained by electron microscopy from core C3.The AFM facility includes a Digital Instruments (DI) Nanoscope IIIa Multimode AFM equipped with a liquid cell and aDI Bioscope IIIa instrument equipped with a liquid cell and mounted on a Zeiss axiovert fluorescence microscope.The former instrument is optimized for force measurements and the latter for imaging, but both instrument are capableof both imaging and force measurements and are capable of operating in either contact or tapping modes.The dynamic light scattering facility consists of two instruments, a DynaPro 99 instrument (Protein Solutions,Charlottesville, VA) housed at the Institute for Medicine and Engineering and a Brookhaven Instruments BI-200SM(Brookhaven, NY) variable angle dynamic light scattering system housed in the Laboratory for Research on theStructure or Matter (LRSM).Studies in project R3 will also be supported by a Kibron Instruments (Helsinki) MicroTrough S-LB lipid monolayersystem equipped with a Layer X deposition accessory that allows deposition of Langrnuir-Blodgett films on solidsupports to enable studies of motor protein-lipid membrane binding in studies coordinated with core C4.The core facilities will be administered by the core leader and a research associate whoexperiments and train members of the research groups in each project.
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