Abstract

Primary and metastatic human tumor tissues are being studied comparatively to determine if differences can be identified, possibly denoting a shift in cell populations in metastatic tumor. In addition, normal tissue samples and serum are being collected as required. The patients have been clinically followed in order to be able to relate any of these parameters with clinical course. To date, a computerized software system has been developed that allows a monthly printout of patient disease status and survival. Input of our population is current to date. The most interesting laboratory developments thus far are: (1) the finding of a reduction in tumor plasminogen activator released into media from cultured metastatic cells versus cultured primary, and (2) the indication that primary tumors which show this reduction are associated with earlier recurrence of disease. A large number of patients have been studied to date, and this appears to be a true finding. In addition, a third finding has been a definite shift in ploidy in some metastatic colon populations as seen by flow cytometry. These three positive findings are being explored and a number of new parameters are being studied. (V)

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA028853-06
Application #
3093251
Study Section
Cancer Therapeutics Program Project Review Committee (CTR)
Project Start
1982-08-01
Project End
1990-03-31
Budget Start
1988-04-01
Budget End
1990-03-31
Support Year
6
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Roswell Park Cancer Institute Corp
Department
Type
DUNS #
City
Buffalo
State
NY
Country
United States
Zip Code
14263

  Publications

Kessler, T L; Markus, G (1991) Epidermal growth factor and 12-tetradecanoyl phorbol 13-acetate induction of urokinase in A431 cells. Semin Thromb Hemost 17:217-24
Radford, D M; Nakai, H; Eddy, R L et al. (1990) Two chromosomal locations for human ornithine decarboxylase gene sequences and elevated expression in colorectal neoplasia. Cancer Res 50:6146-53
Marian, B; Harvey, S; Infante, D et al. (1990) Urokinase secretion from human colon carcinomas induced by endogenous diglycerides. Cancer Res 50:2245-50
Davis, L M; Byers, M G; Fukushima, Y et al. (1988) Four new DNA markers are assigned to the WAGR region of 11p13: isolation and regional assignment of 112 chromosome 11 anonymous DNA segments. Genomics 3:264-71
Davis, L M; Stallard, R; Thomas, G H et al. (1988) Two anonymous DNA segments distinguish the Wilms' tumor and aniridia loci. Science 241:840-2
Harvey, S R; Lawrence, D D; Madeja, J M et al. (1988) Secretion of plasminogen activators by human colorectal and gastric tumor explants. Clin Exp Metastasis 6:431-50
Radford, D M; Eddy, R; Haley, L et al. (1988) Gene sequences coding for S-adenosylmethionine decarboxylase are present on human chromosome 6 and the X and are not amplified in colon neoplasia. Cytogenet Cell Genet 49:285-8
Gerlach, J H; Bell, D R; Karakousis, C et al. (1987) P-glycoprotein in human sarcoma: evidence for multidrug resistance. J Clin Oncol 5:1452-60
Shows, T B; Davis, L M; Qin, S et al. (1986) The chromosome 11 gene map: genes for growth and development, Wilms' tumor deletions, and cancer chromosome breakpoints. Cold Spring Harb Symp Quant Biol 51 Pt 2:867-77
Tricoli, J V; Rall, L B; Karakousis, C P et al. (1986) Enhanced levels of insulin-like growth factor messenger RNA in human colon carcinomas and liposarcomas. Cancer Res 46:6169-73

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