Abstract

The Immunology Core will serve as a critical component of the """"""""Regulation of Anti-Tumor Immunity"""""""" Program, providing services and expertise in both cell isolation and purification and the measurement of antigen specific effector functions. The Core will provide preparative cell isolation using magnetic bead sorting with the autoMACS Separator. The Core will also provide analyses of the frequencies of cells expressing cytokines and other effector molecules (e.g., granzyme) by ELISPOT using the C.T.L. ImmunoSpot automated ELISPOT counter. Lastly, the Core will work with individual investigators on the technical design of cell preparation and animal manipulation and in the interpretation of experimental data.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA109094-04
Application #
8058777
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
4
Fiscal Year
2010
Total Cost
$69,108
Indirect Cost

  Institution

Name
University of Miami School of Medicine
Department
Type
DUNS #
052780918
City
Coral Gables
State
FL
Country
United States
Zip Code
33146

  Publications

Wolf, Dietlinde; Barreras, Henry; Bader, Cameron S et al. (2017) Marked in Vivo Donor Regulatory T Cell Expansion via Interleukin-2 and TL1A-Ig Stimulation Ameliorates Graft-versus-Host Disease but Preserves Graft-versus-Leukemia in Recipients after Hematopoietic Stem Cell Transplantation. Biol Blood Marrow Transplant 23:757-766
Schwartz, Marc; Zhang, Yu; Rosenblatt, Joseph D (2016) B cell regulation of the anti-tumor response and role in carcinogenesis. J Immunother Cancer 4:40
McCormack, Ryan M; Lyapichev, Kirill; Olsson, Melissa L et al. (2015) Enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of Perforin-2. Elife 4:
McCormack, Ryan M; de Armas, Lesley R; Shiratsuchi, Motoaki et al. (2015) Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. Elife 4:
Hatfield, Stephen M; Kjaergaard, Jorgen; Lukashev, Dmitriy et al. (2015) Immunological mechanisms of the antitumor effects of supplemental oxygenation. Sci Transl Med 7:277ra30
Newman, Robert G; Dee, Michael J; Malek, Thomas R et al. (2014) Heat shock protein vaccination and directed IL-2 therapy amplify tumor immunity rapidly following bone marrow transplantation in mice. Blood 123:3045-55
Gonzalez, Louis; Strbo, Natasa; Podack, Eckhard R (2013) Humanized mice: novel model for studying mechanisms of human immune-based therapies. Immunol Res 57:326-34
McCormack, Ryan; de Armas, Lesley R; Shiratsuchi, Motoaki et al. (2013) Inhibition of intracellular bacterial replication in fibroblasts is dependent on the perforin-like protein (perforin-2) encoded by macrophage-expressed gene 1. J Innate Immun 5:185-94
Fields, K A; McCormack, R; de Armas, L R et al. (2013) Perforin-2 restricts growth of Chlamydia trachomatis in macrophages. Infect Immun 81:3045-54
McCormack, Ryan; de Armas, Lesley; Shiratsuchi, Motoaki et al. (2013) Killing machines: three pore-forming proteins of the immune system. Immunol Res 57:268-78

Showing the most recent 10 out of 32 publications