This is the second competing renewal of our Program Project Grant, """"""""Drugs of Abuse: Role of Protein phosphorylation"""""""" (DA 10044), which was initially funded in 1996 and again in 2001. The grant has been highly successful based on research productivity and initiation of new research projects. The overall objective of the Program Project Grant remains the same as the original, namely, to elucidate the molecular basis of the actions of drugs of abuse, particularly psychostimulants, in the dorsal striatum and nucleus accumbens. The addictive properties of drugs of abuse such as psychostimulants depend on their ability to augment dopamine neurotransmission in the basal ganglia. ? ? The Program Project Grant is organized in four Projects, a Scientific Core and an Administrative Core. The Administrative Core staff will support the integration of the researchers and institutions involved in the Program Project Grant. The main goal of the Scientific Core is to provide a research and technical support facility for all of the Program projects. Responsibilities of the Scientific Core will include the creation, characterization, and breeding of genetically altered animals; the design and execution of yeast two-hybrid studies; the production and maintenance of key reagents stocks and new reagents; and the performance of certain routine tasks required to accomplish the studies. Project I, """"""""Psychostimulants and Dendritic spines,"""""""" will focus on the role five regulators of actin dynamics play as targets of psychostimulants in the dendritic spines. Project II, """"""""The Role of DARPP-32, CK1, and CK2 in Mediating the Molecular and Behavioral Effects of Drugs of Abuse,"""""""" will extend previous studies of four key phosphorylation sites of DARPP-32 upon in vivo administration of drugs of abuse and chronic exposure to drugs of abuse. Project III, """"""""Striatal Phosphoproteins and the Actions of Psychostimulants,"""""""" which will be a subcontract carried out at Yale University School of Medicine, study the role(s) of RCS and ARPP-16 in mediating or modulating the actions of psychostimulants as well as study the roles of the three isoforms of PP1 (PP1alpha, beta and gamma) in the actions of psychostimulants. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Program Projects (P01)
Project #
5P01DA010044-12
Application #
7216235
Study Section
Special Emphasis Panel (ZDA1-RXL-E (18))
Program Officer
Satterlee, John S
Project Start
1997-03-01
Project End
2011-01-31
Budget Start
2007-02-01
Budget End
2008-01-31
Support Year
12
Fiscal Year
2007
Total Cost
$1,500,627
Indirect Cost
Name
Rockefeller University
Department
Other Basic Sciences
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
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Musante, Veronica; Li, Lu; Kanyo, Jean et al. (2017) Reciprocal regulation of ARPP-16 by PKA and MAST3 kinases provides a cAMP-regulated switch in protein phosphatase 2A inhibition. Elife 6:
Milosevic, Ana; Liebmann, Thomas; Knudsen, Margarete et al. (2017) Cell- and region-specific expression of depression-related protein p11 (S100a10) in the brain. J Comp Neurol 525:955-975

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