The Neuroanatomy Core provides histology, imaging, and stereotaxic surgery services to several of the projects in the P01, as indicated below. Having a core laboratory perform these services, assures the investigators high quality technical help that is overseen by the Core Director, Dr. Elmquist. We have also found that there is also considerable efficiency gained by buying supplies in large lots and by utilizing the talents of a highly skilled technician on a continuing basis for the tasks he has been trained to perform, which further increases his skill levels. As in the previous cycle, the Core does not have Aims per se, but will provide the services to the projects that are necessary for them to achieve a wide variety of scientific aims, including several Aims in this proposal. This includes tissue sectioning, immunohistochemistry, in situ hybridization histochemistry, and assistance in image analysis and production of figures. In addition, the Core will provide assistance with stereotaxic surgical experiments. The establishment of the Core has facilitated the transition of laboratories that do little histology and neuroanatomy (Lowell, Kahn, Flier) from molecular biology and traditional endocrine assays into the field of functional neuroanatomy, as they can take advantage of the technical proficiency of the Core staff and facilities. In addition, investigators who use similar techniques (e.g. Elmquist and Maratos-Flier) can take advantage of economy of scale of isotopes, emulsions, and technician training for specialized procedures.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Program Projects (P01)
Project #
5P01DK056116-08
Application #
7392813
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2007-04-01
Project End
2010-03-31
Budget Start
2007-04-01
Budget End
2008-03-31
Support Year
8
Fiscal Year
2007
Total Cost
$128,545
Indirect Cost
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215
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Vella, Kristen R; Ramadoss, Preeti; Lam, Francis S et al. (2011) NPY and MC4R signaling regulate thyroid hormone levels during fasting through both central and peripheral pathways. Cell Metab 14:780-90
Loh, Kim; Fukushima, Atsushi; Zhang, Xinmei et al. (2011) Elevated hypothalamic TCPTP in obesity contributes to cellular leptin resistance. Cell Metab 14:684-99
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Xu, Yong; Jones, Juli E; Lauzon, Danielle A et al. (2010) A serotonin and melanocortin circuit mediates D-fenfluramine anorexia. J Neurosci 30:14630-4

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