Abstract

The Specific Aims of the Protein Core are to provide instrumentation, methodological development, experimental training, and technical support in protein microchemistry, protein expression, protein purification, and sample preparation for protein structure determination, as required for the Projects of this Program Project. In the previous project periods, the Protein Core has concentrated on protein microchemistry, including peptide synthesis and protein microsequencing. In the next project period, it is proposed that the protein Core are: 1. To express proteins of interest in signal transduction in the cardiovascular system at high level in bacterial, viral, or yeast expression systems, as needed in the Projects of this Program Project; 2. To efficiently purify proteins expressed in bacterial, viral, or yeast expression systems in high yield, with or without epitope tags, as needed in the Projects of this Program Projects; and 3. To prepare protein samples for determination of three-dimensional structure, including labeling with stable isotopes for NMR analysis and crystallization for x-ray diffraction analysis, as needed in the Projects of this Program Project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL044948-11
Application #
6503025
Study Section
Project Start
1991-05-17
Project End
2006-06-30
Budget Start
Budget End
Support Year
11
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Kaufmann, Susann G; Westenbroek, Ruth E; Maass, Alexander H et al. (2013) Distribution and function of sodium channel subtypes in human atrial myocardium. J Mol Cell Cardiol 61:133-141
Westenbroek, Ruth E; Bischoff, Sebastian; Fu, Ying et al. (2013) Localization of sodium channel subtypes in mouse ventricular myocytes using quantitative immunocytochemistry. J Mol Cell Cardiol 64:69-78
Marshall, Misty R; Clark 3rd, John Patrick; Westenbroek, Ruth et al. (2011) Functional roles of a C-terminal signaling complex of CaV1 channels and A-kinase anchoring protein 15 in brain neurons. J Biol Chem 286:12627-39
Fu, Ying; Westenbroek, Ruth E; Yu, Frank H et al. (2011) Deletion of the distal C terminus of CaV1.2 channels leads to loss of beta-adrenergic regulation and heart failure in vivo. J Biol Chem 286:12617-26
Reiner, Cindy L; McCullar, Jennifer S; Kow, Rebecca L et al. (2010) RACK1 associates with muscarinic receptors and regulates M(2) receptor trafficking. PLoS One 5:e13517
Brunet, Sylvain; Scheuer, Todd; Catterall, William A (2009) Cooperative regulation of Ca(v)1.2 channels by intracellular Mg(2+), the proximal C-terminal EF-hand, and the distal C-terminal domain. J Gen Physiol 134:81-94
Heikaus, Clemens C; Pandit, Jayvardhan; Klevit, Rachel E (2009) Cyclic nucleotide binding GAF domains from phosphodiesterases: structural and mechanistic insights. Structure 17:1551-1557
Santana, Luis F; Navedo, Manuel F; Amberg, Gregory C et al. (2008) Calcium sparklets in arterial smooth muscle. Clin Exp Pharmacol Physiol 35:1121-6
Martinez, Sergio E; Heikaus, Clemens C; Klevit, Rachel E et al. (2008) The structure of the GAF A domain from phosphodiesterase 6C reveals determinants of cGMP binding, a conserved binding surface, and a large cGMP-dependent conformational change. J Biol Chem 283:25913-9
Nathanson, Neil M (2008) Synthesis, trafficking, and localization of muscarinic acetylcholine receptors. Pharmacol Ther 119:33-43

Showing the most recent 10 out of 104 publications