Abstract

The scientific core consists of two major imaging laboratories to support program project members with state of the art equipment and methodologies.
Aim 1. Provide support to investigators to design and carry out anatomically based analyses utilizing quantitative neuroanatomical techniques including stereology based statistically unbiased approaches.
Aim 2. Assist investigators in imaging blood brain barrier breakdown and MMP uregulation, both in vivo and ex vivo, using extravasation of fluorescent indicators.
Aim 3. Assist investigators in utilizing laser speckle contrast imaging in studies of cerebral blood flow and in the application of multi-spectral in-vivo imaging of intrinsic (e.g hemoglobin concentrations) and extrinsic (e.g. plasma leakiness indicators and MMP activatable fluorescence markers) contrast agents. Advance the methodology to provide more robust measures of blood flow and facilitate investigator driven advanced analyses.
Aim 4. Develop robust high-field MRI measurements of CBF in rodents, and assist investigators in the acquisition and analysis of anatomical and functional MRI data. Taken together, these aims both support the scientific aims of the program project as a whole, while the needs of the projects serve to advance the methodology to facilitate investigator driven analyses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS010828-33
Application #
7615142
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2008-05-01
Budget End
2009-04-30
Support Year
33
Fiscal Year
2008
Total Cost
$252,840
Indirect Cost

  Institution

Name
Massachusetts General Hospital
Department
Type
DUNS #
073130411
City
Boston
State
MA
Country
United States
Zip Code
02199

  Publications

Miao, Yanying; Liao, James K (2014) Potential serum biomarkers in the pathophysiological processes of stroke. Expert Rev Neurother 14:173-85
Sawada, Naoki; Liao, James K (2014) Rho/Rho-associated coiled-coil forming kinase pathway as therapeutic targets for statins in atherosclerosis. Antioxid Redox Signal 20:1251-67
Tanaka, Shin-ichi; Fukumoto, Yoshihiro; Nochioka, Kotaro et al. (2013) Statins exert the pleiotropic effects through small GTP-binding protein dissociation stimulator upregulation with a resultant Rac1 degradation. Arterioscler Thromb Vasc Biol 33:1591-600
Montalvo, J; Spencer, C; Hackathorn, A et al. (2013) ROCK1 & 2 perform overlapping and unique roles in angiogenesis and angiosarcoma tumor progression. Curr Mol Med 13:205-19
Hung, Ming-Jui; Cherng, Wen-Jin; Hung, Ming-Yow et al. (2012) Increased leukocyte Rho-associated coiled-coil containing protein kinase activity predicts the presence and severity of coronary vasospastic angina. Atherosclerosis 221:521-6
Wang, Chao-Yung; Liao, James K (2012) A mouse model of diet-induced obesity and insulin resistance. Methods Mol Biol 821:421-33
Zhou, Qian; Mei, Yu; Shoji, Takuhito et al. (2012) Rho-associated coiled-coil-containing kinase 2 deficiency in bone marrow-derived cells leads to increased cholesterol efflux and decreased atherosclerosis. Circulation 126:2236-47
Wang, Qing Mei; Stalker, Timothy J; Gong, Yulan et al. (2012) Inhibition of Rho-kinase attenuates endothelial-leukocyte interaction during ischemia-reperfusion injury. Vasc Med 17:379-85
Wang, Qing Mei; Liao, James K (2012) ROCKs as immunomodulators of stroke. Expert Opin Ther Targets 16:1013-25
Liao, James K (2012) Mitohormesis: another pleiotropic effect of statins? Eur Heart J 33:1299-301

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