Abstract

The purpose of this study is to understand the physiologic and morphologic changes which occur when human laryngeal epithelium forms papillomas, and the etiology of these changes. From this information, it may be possible to develop better modes of treatment for the papillomatous diseases. The following studies will be done: Changes in voice prints and aerodynamic flow loops following surgical removal or other therapeutic treatment of papillomas will be evaluated. The morphology of benign and malignant papillomas will be studied at the level of the light and electron microscopes. Papillomas will be cultured in vitro and their response to changes in culture conditions will be compared to normal epithelium. Tissues and cultured cells will be analyzed for the presence of Human Papilloma Virus (HPV) DNA. Normal cells will be infected with HPV to transform them in vitro to the papilloma phenotype. Hormone receptors on the surface of normal and papillomatous cells will be characterized. Papilloma-specific proteins will be studied by preparing monoclonal antibodies against papilloma chromatin, which will then be used to study early events in the formation of papilloma lesions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS019214-03
Application #
3099739
Study Section
Communicative Disorders Review Committee (CDR)
Project Start
1983-08-01
Project End
1986-07-31
Budget Start
1985-08-01
Budget End
1986-07-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Long Island Jewish Medical Center
Department
Type
DUNS #
City
New Hyde Park
State
NY
Country
United States
Zip Code
11040

  Publications

Abramson, A L; DiLorenzo, T P; Steinberg, B M (1990) Is papillomavirus detectable in the plume of laser-treated laryngeal papilloma? Arch Otolaryngol Head Neck Surg 116:604-7
Steinberg, B M; Meade, R; Kalinowski, S et al. (1990) Abnormal differentiation of human papillomavirus-induced laryngeal papillomas. Arch Otolaryngol Head Neck Surg 116:1167-71
DiLorenzo, T P; Steinberg, B M (1990) Laryngeal keratinocytes show variable inhibition of replication by TGF-beta. J Cell Sci 96 ( Pt 1):115-9
Di Lorenzo, T P; De Maro, J A; Pumo, D E (1989) Nutritional requirements of papillomavirus-transformed mouse cells and an uninfected parent line in serum-free culture. In Vitro Cell Dev Biol 25:909-13
Brandwein, M; Steinberg, B; Thung, S et al. (1989) Human papillomavirus 6/11 and 16/18 in Schneiderian inverted papillomas. In situ hybridization with human papillomavirus RNA probes. Cancer 63:1708-13
Steinberg, B M; Auborn, K J; Brandsma, J L et al. (1989) Tissue site-specific enhancer function of the upstream regulatory region of human papillomavirus type 11 in cultured keratinocytes. J Virol 63:957-60
Steinberg, B M; Gallagher, T; Stoler, M et al. (1988) Persistence and expression of human papillomavirus during interferon therapy. Arch Otolaryngol Head Neck Surg 114:27-32
Shikowitz, M J; Steinberg, B M; Galli, R L et al. (1988) Histological and molecular analysis of cottontail rabbit papillomavirus-induced papillomas treated with hematoporphyrin derivative photodynamic therapy. Arch Otolaryngol Head Neck Surg 114:175-8
Zaslav, A L; Steinberg, B M; Stamberg, J et al. (1988) Chromosomal analysis of recurrent laryngeal papillomas. Arch Otolaryngol Head Neck Surg 114:1170-4
Mullooly, V M; Abramson, A L; Steinberg, B M et al. (1988) Clinical effects of alpha-interferon dose variation on laryngeal papillomas. Laryngoscope 98:1324-9

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