This is a proposal to establish a Center of Excellence combining the strengths of three large laboratories, each of which is currently approaching major problems of developmental neurobiology at a different level. The primary aim of the project is to develop a concerted, synergistic attack on several major developmental problems: neural cell interactions and regeneration, neuronal viability based on synaptic interactions, and mapping of sensory functions. Each of the laboratories has already developed methodological salients for attacking these problems: molecular cellular adhesion assays, phosphoprotein characterization, tissue culture assays, and electrophysiology and neuroanatomic mapping. By combining these intellectual and operational resources, it is expected that major progress can be made using a multilevel attack and defined systems shared among all three research groups. At the molecular level, we will analyze the expression of cell adhesion molecules in regeneration of neural and neuromuscular systems and in associated cell migration events and the order of gene expression of the phosphoproteins synapsin I, DARPP-32 and G-substrate as a function of synaptogenesis, at the cellular level, we will investigate the laminar, columnar and connectional organization in neural maps instructing such as the striate cortex, using identification of cell specific surface markers and in vitro tissue slice techniques. At the highest level, the contribution of these processes to physiological function will be assessed using electrophysiological measurements. The successful merger of these approaches will require the combined efforts and cooperation of these three laboratories which are in close physical proximity. A Center of Excellence would strongly enhance their intellectual cooperation and the results of this merger are expected to have direct pertinence to the study of degenerative disease and neural regeneration.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS022789-04
Application #
3099948
Study Section
(SRC)
Project Start
1985-09-01
Project End
1990-08-31
Budget Start
1988-09-01
Budget End
1989-08-31
Support Year
4
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Rockefeller University
Department
Type
Graduate Schools
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
Murakoshi, T (1995) Cholinergic modulation of synaptic transmission in the rat visual cortex in vitro. Vision Res 35:25-35
Edelman, G M (1994) Adhesion and counteradhesion: morphogenetic functions of the cell surface. Prog Brain Res 101:1-14
Yagi, T; Macleish, P R (1994) Ionic conductances of monkey solitary cone inner segments. J Neurophysiol 71:656-65
Shiga, T; Shirai, T; Grumet, M et al. (1993) Differential expression of neuron-glia cell adhesion molecule (Ng-CAM) on developing axons and growth cones of interneurons in the chick embryo spinal cord: an immunoelectron microscopic study. J Comp Neurol 329:512-8
Hirsch, J A; Gilbert, C D (1993) Long-term changes in synaptic strength along specific intrinsic pathways in the cat visual cortex. J Physiol 461:247-62
Burgoon, M P; Grumet, M; Mauro, V et al. (1991) Structure of the chicken neuron-glia cell adhesion molecule, Ng-CAM: origin of the polypeptides and relation to the Ig superfamily. J Cell Biol 112:1017-29
Hirsch, J A; Gilbert, C D (1991) Synaptic physiology of horizontal connections in the cat's visual cortex. J Neurosci 11:1800-9
Gilbert, C D; Hirsch, J A; Wiesel, T N (1990) Lateral interactions in visual cortex. Cold Spring Harb Symp Quant Biol 55:663-77
Prieto, A L; Jones, F S; Cunningham, B A et al. (1990) Localization during development of alternatively spliced forms of cytotactin mRNA by in situ hybridization. J Cell Biol 111:685-98
Shiga, T; Oppenheim, R W; Grumet, M et al. (1990) Neuron-glia cell adhesion molecule (Ng-CAM) expression in the chick embryo spinal cord: observations on the earliest developing intersegmental interneurons. Brain Res Dev Brain Res 55:209-17

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