Intense research efforts over the past four decades have provided a wealth of information on the development, structure, and function of the cerebellum. Still there remain many outstanding issues on the role of the cerebellum in motor learning and its neuronal substrate, the spatial and temporal interactions between climbing and mossy fiber afferent systems, and the relations between neuronal activity and cerebellar blood flow. Also there remain many gaps in our understanding of the molecular events governing cerebellar neuronal development and on the specific glutamate receptor subtypes associated with the neural input to Purkinje cells. Obtaining answers to these questions is critical for defining the basic events underlying cerebellar development and functional organization and for obtaining insights into the mechanism leading to cerebellar dysfunction. This program project application builds upon the strong interactions and productive collaborations established among the investigators during the last funding period to continue to explore cerebellar gene expression, neuronal development, neurochemistry and function. The investigators use state-of-the-art molecular, cellular and system approaches to address hypothesis concerning: 1) the role of the CREB family of transcription factors system in Purkinje cell synaptic plasticity; 2) the interaction between cell cycle regulatory neuronal differentiation, 3) glutamate receptor subtypes associated with the molecular layer of the cerebellum and their involvement in nitric oxide production; 4) neural mechanisms mediating changes in cerebellar blood flow during normal cerebellar function; and 5) the spatial and temporal characteristics and interactions of the climbing and mossy fiber afferents. This Program Project offers a unique and highly integrated multi-disciplinary approach to continue to provide new information on fundamental aspects of cerebellar development, structure and function.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS031318-09
Application #
6539751
Study Section
Special Emphasis Panel (ZNS1-SRB-W (01))
Program Officer
Chen, Daofen
Project Start
1993-07-01
Project End
2004-03-31
Budget Start
2002-04-01
Budget End
2003-03-31
Support Year
9
Fiscal Year
2002
Total Cost
$900,875
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Kuceyeski, Amy; Navi, Babak B; Kamel, Hooman et al. (2016) Structural connectome disruption at baseline predicts 6-months post-stroke outcome. Hum Brain Mapp 37:2587-601
Chen, Gang; Gao, Wangcai; Reinert, Kenneth C et al. (2005) Involvement of kv1 potassium channels in spreading acidification and depression in the cerebellar cortex. J Neurophysiol 94:1287-98
Goswami, Jaideep; Martin, Loren A; Goldowitz, Daniel et al. (2005) Enhanced Purkinje cell survival but compromised cerebellar function in targeted anti-apoptotic protein transgenic mice. Mol Cell Neurosci 29:202-21
Brodie, Christopher R; Khaliq, Mahmooda; Yin, Jerry C P et al. (2004) Overexpression of CREB reduces CRE-mediated transcription: behavioral and cellular analyses in transgenic mice. Mol Cell Neurosci 25:602-11
Reinert, Kenneth C; Dunbar, Robert L; Gao, Wangcai et al. (2004) Flavoprotein autofluorescence imaging of neuronal activation in the cerebellar cortex in vivo. J Neurophysiol 92:199-211
Dunbar, R L; Chen, G; Gao, W et al. (2004) Imaging parallel fiber and climbing fiber responses and their short-term interactions in the mouse cerebellar cortex in vivo. Neuroscience 126:213-27
Beitz, Alvin J; Saxon, Dale (2004) Harmaline-induced climbing fiber activation causes amino acid and peptide release in the rodent cerebellar cortex and a unique temporal pattern of Fos expression in the olivo-cerebellar pathway. J Neurocytol 33:49-74
Zhang, Yi; Forster, Colleen; Milner, Teresa A et al. (2003) Attenuation of activity-induced increases in cerebellar blood flow by lesion of the inferior olive. Am J Physiol Heart Circ Physiol 285:H1177-82
Yang, Guang; Zhang, Yi; Ross, M Elizabeth et al. (2003) Attenuation of activity-induced increases in cerebellar blood flow in mice lacking neuronal nitric oxide synthase. Am J Physiol Heart Circ Physiol 285:H298-304
Gao, Wangcai; Dunbar, Robert L; Chen, Gang et al. (2003) Optical imaging of long-term depression in the mouse cerebellar cortex in vivo. J Neurosci 23:1859-66

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