Abstract

The DNA Sequencing Shared Resource carries out centralized DNA sequencing for Cancer Center members. The availability of on-site DNA sequencing is of central importance to Cancer Center members. Local DNA sequencing allows for rapid turn-around, and with CCSG support, the cost is competitive with off-site commercial facilities. The DNA Sequencing Shared Resource contains an ABI 3700 automated capillary DNA sequencer, a Beckman DNA oligomer synthesizer, and a Robbins Scientific Hydra-96 microdispenser workstation. Computer support for the Shared Resource is provided from the adjoining laboratory of the core Director Moyzis. The DNA Sequencing Shared Resource sequences DNA samples that have been prepared by the investigators. The dried samples are processed by the Shared Resource and sequenced on the ABI 3700 sequencer. The sequence data is deposited as an FTP file on the Shared Resource website. Each investigator signs in and transfers the FTP file containing the sequencer output for his/her DNA samples. The automated and streamlined process allows for rapid and economic ($2.50 per sample) sequencing of DNA samples. The DNA sequencing Shared Resource will be moved to Sprague in the spring of 2002, in close proximity to the Microarray Shared Resource, and a bioinformatics facility. This will enhance the effectiveness of the Shared Resource.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA062203-09
Application #
6683021
Study Section
Subcommittee E - Prevention &Control (NCI)
Project Start
2002-09-19
Project End
2007-01-31
Budget Start
Budget End
Support Year
9
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of California Irvine
Department
Type
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Sierra, Robert A; Hoverter, Nathan P; Ramirez, Ricardo N et al. (2018) TCF7L1 suppresses primitive streak gene expression to support human embryonic stem cell pluripotency. Development 145:
Maciejewski, Sonia; Ullmer, Wendy; Semler, Bert L (2018) VPg unlinkase/TDP2 in cardiovirus infected cells: Re-localization and proteolytic cleavage. Virology 516:139-146
Konstorum, Anna; Lowengrub, John S (2018) Activation of the HGF/c-Met axis in the tumor microenvironment: A multispecies model. J Theor Biol 439:86-99
Yan, Huaming; Konstorum, Anna; Lowengrub, John S (2018) Three-Dimensional Spatiotemporal Modeling of Colon Cancer Organoids Reveals that Multimodal Control of Stem Cell Self-Renewal is a Critical Determinant of Size and Shape in Early Stages of Tumor Growth. Bull Math Biol 80:1404-1433
Flather, Dylan; Nguyen, Joseph H C; Semler, Bert L et al. (2018) Exploitation of nuclear functions by human rhinovirus, a cytoplasmic RNA virus. PLoS Pathog 14:e1007277
Wang, Xiaolin; Zhao, Da; Phan, Duc T T et al. (2018) A hydrostatic pressure-driven passive micropump enhanced with siphon-based autofill function. Lab Chip 18:2167-2177
George, Andrée S; Cox, Clayton E; Desai, Prerak et al. (2018) Interactions of Salmonella enterica Serovar Typhimurium and Pectobacterium carotovorum within a Tomato Soft Rot. Appl Environ Microbiol 84:
Hou, Jue; Williams, Joshua; Botvinick, Elliot L et al. (2018) Visualization of Breast Cancer Metabolism Using Multimodal Nonlinear Optical Microscopy of Cellular Lipids and Redox State. Cancer Res 78:2503-2512
Lagarrigue, Frederic; Gingras, Alexandre R; Paul, David S et al. (2018) Rap1 binding to the talin 1 F0 domain makes a minimal contribution to murine platelet GPIIb-IIIa activation. Blood Adv 2:2358-2368
Reidling, Jack C; Relaño-Ginés, Aroa; Holley, Sandra M et al. (2018) Human Neural Stem Cell Transplantation Rescues Functional Deficits in R6/2 and Q140 Huntington's Disease Mice. Stem Cell Reports 10:58-72

Showing the most recent 10 out of 1106 publications