Imaging of tissues and isolated cells is a critical tool for determining the biochemical and molecularmechanisms involved in phenotypic and functional changes in tissues, cells, and subcellular organellesinduced by exposure to xenobiotics. Recent advances in imaging, biological chemistry, and molecularbiology, have led to an escalation in imaging technologies and use in the biomedical sciences over thelast several years. Development of fluorescent probes, green fluorescent protein (GFP), confocal andtwo-photon optics, and photoelectronic detectors, have allowed the visualization of single molecules,monitoring of molecular interactions in live cells, and enhanced ability to image complex tissues. Thecomplexity, time, and cost required for developing state-of-the-art imaging technologies makes itunfeasible for individual investigators to independently obtain these important tools and approaches forbiomedical research. Therefore, the need exists for an organized group of specialists with the expertisein imaging technologies to acquire the instrumentation, master the applications, and assist individualinvestigators in using the technology to answer specific research questions.The long-term goal of the Cell Imaging and Analysis Facility is to provide access to state-of-the-artinstrumentation and expertise in flow cytometry and cell sorting, histopathology, and microscopy. Ourobjective is to develop fluorescence-based technologies to meet the current and future needs of Centerinvestigators. Our rationale is that access to state-of-the-art fluorescence technologies will allow Centerinvestigators to determine specific mechanisms of xenobiotic action at the cellular level. The motivatedand interactive staff of the facility provides expertise in complementary areas of imaging and analysis. Dr.Kerkvliet and Ms. Oughton have over 40 years combined experience in flow cytometry with anoutstanding record of research productivity. Dr. Greenwood and Ms. Fraley have recently setup theconfocal and widefield microscopy systems, publishing studies resulting from the development offluorescence recovery after photobleaching and fluorescence resonance energy transfer microscopy. Dr.Lohr is a certified Veterinary Pathologist with important collaborative interactions with Centerinvestigators demonstrated by recent publications and Ms. Fischer has over 20 years of experience inhistology, image analysis, and electron microscopy. Lastly, our collaboration with MolecularProbes/lnvitrogen, providing access to reagents and expertise, makes us especially well-positioned toaccomplish our objective.
Showing the most recent 10 out of 690 publications
