Mass Spectrometric Studies of Integral Membrane Proteins & Ion Channels

MacKinnon, Roderick

Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We continue to develop the utility of mass spectrometry to assist in the definition of integral membrane proteins and (especially ion channels) at atomic resolutions using diffraction methods. A number of publications have resulted from this work D.A. Doyle, J.M. Cabral, R.A. Pfuetzner, A. Kuo, J.M. Gulbis, S.L. Cohen, B.T. Chait, R. MacKinnon, """"""""The Structure of the Potassium Channel: Molecular Basis of K+ Conduction and Selectivity"""""""" Science 280, (1998) 69-77. R. MacKinnon, S.L. Cohen, A. Kuo, A. Lee, B.T. Chait """"""""Structural Conservation in Prokaryotic and Eukaryotic Potassium Channels"""""""" Science 280 (1998) 106-109. J.H. Morais Cabral, A. Lee, S. Cohen, B.T. Chait, M. Li, R. MacKinnon """"""""Structure of the HERG potassium channel amino-terminal domain: definition of a structural family of PAS domains"""""""" Cell 95 (1998) 649-655. J.M. Gulbis, M. Zhou, S. Mann, R. MacKinnon """"""""Structure of the Cytoplasmic ( Subunit-T1 Assembly of Voltage-Dependent K+ Channels"""""""" Science 289(2000) 123-127. M. Cadene, B.T. Chait """"""""A Robust Detergent-Friendly for Mass Spectrometric Analysis of Integral Membrane Proteins"""""""" Anal. Chem. 72 (2000) 5655-5658. S.L. Cohen, B.T. Chait """"""""Mass Spectrometry as a tool for Studying Protein Structure"""""""" Ann. Reviews of Biophysics and Biomolecular Structure 30 (2001) 67-85. R. Dutzler, ER Campbell, M. Cadene, B.T. Chait, R.MacKinnon """"""""X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity"""""""" Nature 415 (2002) 287-94. Y. Jiang, A. Lee, J. Chen, M. Cadene, B. T. Chait, R. MacKinnon """"""""Crystal structure and mechanism of a calcium-gated potassium channel"""""""" Nature 417 (2002) 515-522. Y. Jiang, A. Lee, J. Chen, M. Cadene, B. T. Chait, R. MacKinnon """"""""The open pore conformation of potassium channels"""""""" Nature 417 (2002) 523-526. Y. Jiang, A. Lee, J. Chen, V. Ruta, M. Cadene, B.T. Chait &Roderick MacKinnon """"""""X-ray structure of a voltage-dependent K+ channel"""""""" Nature 423 (2003) 33-41;280. M. Nishida1, M. Cadene, B.T. Chait, R. MacKinnon """"""""Crystal structure of a Kir3.1-prokaryotic Kir channel chimera"""""""" EMBO Journal, 26 (2007) 4005-4015

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Center for Research Resources (NCRR)
• Type: Biotechnology Resource Grants (P41)
• Project #: 5P41RR000862-38
• Application #: 8361483
• Study Section: Special Emphasis Panel (ZRG1-BCMB-Q (40))
• Project Start: 2011-03-01
• Project End: 2012-03-31
• Budget Start: 2011-03-01
• Budget End: 2012-03-31
• Support Year: 38
• Fiscal Year: 2011
• Total Cost: $3,911

Institution

• Name: Rockefeller University
• Department: Miscellaneous
• Type: Other Domestic Higher Education
• DUNS #: 071037113
• City: New York
• State: NY
• Country: United States
• Zip Code: 10065

Publications

Manning, Lois R; Popowicz, Anthony M; Padovan, Julio C et al. (2017) Gel filtration of dilute human embryonic hemoglobins reveals basis for their increased oxygen binding. Anal Biochem 519:38-41

Boice, Michael; Salloum, Darin; Mourcin, Frederic et al. (2016) Loss of the HVEM Tumor Suppressor in Lymphoma and Restoration by Modified CAR-T Cells. Cell 167:405-418.e13

Chait, Brian T; Cadene, Martine; Olinares, Paul Dominic et al. (2016) Revealing Higher Order Protein Structure Using Mass Spectrometry. J Am Soc Mass Spectrom 27:952-65

Krutchinsky, Andrew N; Padovan, Júlio C; Cohen, Herbert et al. (2015) Maximizing ion transmission from atmospheric pressure into the vacuum of mass spectrometers with a novel electrospray interface. J Am Soc Mass Spectrom 26:649-58

Mast, Fred D; Rachubinski, Richard A; Aitchison, John D (2015) Signaling dynamics and peroxisomes. Curr Opin Cell Biol 35:131-6

Krutchinsky, Andrew N; Padovan, Júlio C; Cohen, Herbert et al. (2015) Optimizing electrospray interfaces using slowly diverging conical duct (ConDuct) electrodes. J Am Soc Mass Spectrom 26:659-67

Oricchio, Elisa; Papapetrou, Eirini P; Lafaille, Fabien et al. (2014) A cell engineering strategy to enhance the safety of stem cell therapies. Cell Rep 8:1677-1685

Zhong, Yu; Morris, Deanna H; Jin, Lin et al. (2014) Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels. J Biol Chem 289:26021-37

Xue, John Z; Woo, Eileen M; Postow, Lisa et al. (2013) Chromatin-bound Xenopus Dppa2 shapes the nucleus by locally inhibiting microtubule assembly. Dev Cell 27:47-59

Indiani, Chiara; O'Donnell, Mike (2013) A proposal: Source of single strand DNA that elicits the SOS response. Front Biosci (Landmark Ed) 18:312-23