Biofilm formation has been linked to many chronic bacterial infections. Thus, significant research has been directed towards understanding the basic biology behind biofilm formation. Biofilms produce an extracellular matrix that functions, in part, to hold the community together. Pseudomonas aeruginosa represents a paradigm species for the study of biofilms in the laboratory. The regulation of matrix production and the carbohydrate component of the matrix have both been examined. However, the protein component of the biofilm matrix has been relatively understudied. Our groups identified a biofilm matrix protein for P. aeruginosa, CdrA. CdrA provides structural integrity through extracellular interactions with the matrix polysaccharide Psl. Outside of matrix proteins that provide structural functions, we predict that matrix associated proteins can play both nutritional and protective roles for the community. This project will initially focus on CdrA and its role in the matrix. We will then characterize ecotin a matrix bound protease inhibitor and three matrix-associated proteases. Finally, we will identify new matrix proteins that associate with eps component of the biofilm matrix.

Public Health Relevance

Biofilm formation has been linked to many chronic bacterial infections. Biofilm matrix proteins are an understudied feature of these communities. This project will initially focus on CdrA and its role in the Pseudomonas aeruginosa biofilm matrix and then novel biofilm matrix proteins and investigate their impact biofilm survival and pathogenicity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI143916-03
Application #
10102191
Study Section
Bacterial Pathogenesis Study Section (BACP)
Program Officer
Ernst, Nancy L
Project Start
2019-02-22
Project End
2024-01-31
Budget Start
2021-02-01
Budget End
2022-01-31
Support Year
3
Fiscal Year
2021
Total Cost
Indirect Cost
Name
University of Washington
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195