Luminal Acidification of Epididymis & Vas Deferens

Brown, D.

Abstract

OUR STUDIES ON THE ACIDIFICATION OF THE VAS DEFERENS FORMED ROUND THE COMMON INTEREST OF DRS. SMITH AND BROWN IN THE PHYSIOLOGICAL ROLE OF THE VACUOLAR-TYPE PROTON ATPASE. RESULTS HAVE BEEN EXTREMELY ENCOURAGING WITH PROBE DATA SUPPORTING IMMUNOHISTOCHEMICAL STUDIES IN ATTRIBUTING A MAJOR ROLE TO THESE PUMPS IN REGULATING MAMMALIAN SPERM ACTIVITY. THUS, THE V-TYPE PROTON PUMPING ATPASE IS IMPLICATED AS THE MAJOR SOURCE OF THE LUMINAL ACIDIFICATION IN PARTS OF THE MALE REPRODUCTIVE TRACT. THIS ENZYME IS LOCATED IN A MINORITY CELL TYPE, WHICH RESEMBLES THE ACID-SECRETING INTERCALATED CELL OF THE RENAL COLLECTING DUCT. FURTHER DETAILS ARE GIVEN IN HIGHLIGHTS. THIS PROJECT HAS NOW BEEN JOINED BY DR. BRETON AS POSTDOCTORAL FELLOW IN DR. BROWN~S LAB.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Center for Research Resources (NCRR)
• Type: Biotechnology Resource Grants (P41)
• Project #: 3P41RR001395-14S1
• Application #: 5223336
• Support Year: 14
• Fiscal Year: 1996

Publications

Demidenko, Eugene; Glaholt, S P; Kyker-Snowman, E et al. (2017) Single toxin dose-response models revisited. Toxicol Appl Pharmacol 314:12-23

Chowanadisai, Winyoo; Messerli, Shanta M; Miller, Daniel H et al. (2016) Cisplatin Resistant Spheroids Model Clinically Relevant Survival Mechanisms in Ovarian Tumors. PLoS One 11:e0151089

De Martino, Federico; Moerel, Michelle; Ugurbil, Kamil et al. (2015) Less noise, more activation: Multiband acquisition schemes for auditory functional MRI. Magn Reson Med 74:462-7

Van Mooy, Benjamin A S; Hmelo, Laura R; Fredricks, Helen F et al. (2014) Quantitative exploration of the contribution of settlement, growth, dispersal and grazing to the accumulation of natural marine biofilms on antifouling and fouling-release coatings. Biofouling 30:223-36

Brodsky, Alexander S; Fischer, Andrew; Miller, Daniel H et al. (2014) Expression profiling of primary and metastatic ovarian tumors reveals differences indicative of aggressive disease. PLoS One 9:e94476

De Martino, Federico; Zimmermann, Jan; Muckli, Lars et al. (2013) Cortical depth dependent functional responses in humans at 7T: improved specificity with 3D GRASE. PLoS One 8:e60514

De Martino, Federico; Moerel, Michelle; van de Moortele, Pierre-Francois et al. (2013) Spatial organization of frequency preference and selectivity in the human inferior colliculus. Nat Commun 4:1386

Vang, Souriya; Wu, Hsin-Ta; Fischer, Andrew et al. (2013) Identification of ovarian cancer metastatic miRNAs. PLoS One 8:e58226

Chowanadisai, Winyoo; Graham, David M; Keen, Carl L et al. (2013) Neurulation and neurite extension require the zinc transporter ZIP12 (slc39a12). Proc Natl Acad Sci U S A 110:9903-8

Lobikin, Maria; Chernet, Brook; Lobo, Daniel et al. (2012) Resting potential, oncogene-induced tumorigenesis, and metastasis: the bioelectric basis of cancer in vivo. Phys Biol 9:065002