This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.By the request of David Budil, we undertook the experimental study of a previously proposed design architecture for a novel peptide based nano-actuator. Budil's design is based on the -helical coiled coil portion of the yeast transcriptional activator GCN4, and it has been engineered to obtain an environmentally-responsive nano-actuator. The dimeric coiled-coil peptide consists of two identical ~4.5nm long and ~3 nm wide polypeptide chains. The actuation mechanism depends on the modification of electrostatic charges on histidine residues introduced at various points along the peptide, which is achieved by varying the pH of the solution, Previous molecular dynamic calculations have identified a set of peptide sequences that are expected to adopt the coiled-coil configuration and to exhibit reversible opening as a function of ambient pH. To test these predictions experimentally, the sequences were synthesized with rigid TOAC spin labels attached near the ends of the helices and the distribution of distances between the two labels determined by DEER spectroscopy as a function of sample conditions.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR016292-08
Application #
7724013
Study Section
Special Emphasis Panel (ZRG1-BCMB-K (40))
Project Start
2008-09-01
Project End
2009-08-31
Budget Start
2008-09-01
Budget End
2009-08-31
Support Year
8
Fiscal Year
2008
Total Cost
$2,976
Indirect Cost
Name
Cornell University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
872612445
City
Ithaca
State
NY
Country
United States
Zip Code
14850
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Yu, Renyuan Pony; Darmon, Jonathan M; Hoyt, Jordan M et al. (2012) High-Activity Iron Catalysts for the Hydrogenation of Hindered, Unfunctionalized Alkenes. ACS Catal 2:1760-1764
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Dzikovski, Boris; Tipikin, Dmitriy; Freed, Jack (2012) Conformational distributions and hydrogen bonding in gel and frozen lipid bilayers: a high frequency spin-label ESR study. J Phys Chem B 116:6694-706

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