Acute respiratory distress syndrome (ARDS) is a form of acute respiratory failure resulting from a variety of insults including sepsis, smoke inhalation and severe trauma. ARDS has a high mortality rate of 30-40%, which results in approximately 75,000 deaths per year. This exceeds the mortality due to breast or prostate cancer. Treatment of ARDS is based on supportive mechanical ventilation that is applied while the underlying cause of respiratory failure hopefully resolves. However, selecting appropriate ventilation parameters is difficult because of the conflicting requirements imposed by the inhomogeneous nature of lung injury in ARDS. Inspiratory pressures must be sufficiently low to avoid over-distention of the delicate parenchyma (volutrauma) while at the same time expiratory pressures must be high enough to prevent damage caused by the repetitive collapse (derecruitment) and reopening (recruitment) of airways and alveoli (atelectrauma). Volutrauma and atelectrauma can both lead to ventilator-induced lung injury (VILI) which is manifest as local accumulation of edema in the airspaces. This, in turn, leads to surfactant inactivation, increased tissue stress, and further VILI in a positive feedback mechanism that often leads to death. However, exactly how VILI begins within the lung tissue, and then develops over time, remains poorly understood. We hypothesize that edema and atelectasis begin locally in regions of high tissue stress and then propagate outward to consume the rest of the lung as a result of fluid-structure interactions. This is exacerbated during mechanical ventilation because ventilation heterogeneity amplifies the damage generated in local stress foci. We will test this hypothesis by using design- based stereology to quantify how the spatial distributions of edema and atelectasis change with time during the progression of VILI in mouse models of ARDS. These measurements will then inform the development of a computational model of an alveolar network that couples solid and fluid mechanics to determine how inhomogeneous edema alters microscale tissue stress and recruitment/derecruitment. The numerical model will be used to investigate potentially protective modes of mechanical ventilation, such as variable tidal volume ventilation, that avoid persistently concentrating stress in fixed regions of the lung tissue, as tends to occur with conventional regular ventilation. These studies will facilitate the development of novel protective ventilation strategies for ARDS and thereby help reduce mortality. The PI of this proposal has extensive experience with numerical modeling, animal experimentation, and organ-scale physiology. Complementary training in morphometric analysis will provide the PI with the skills necessary to quantify the micro-scale effects of lung injury, and to link these structural changes to lung function and injury progression using computational models. This program of study and research, together with the world-class research environment provided by the University of Vermont College of Medicine, will enable the PI to develop a career as an independent investigator applying bioengineering and computational methods to the study of lung disease.

Public Health Relevance

Acute respiratory distress syndrome (ARDS) causes more deaths per year than breast or prostate cancer. Treatment for ARDS is based around supportive mechanical ventilation, but this can cause ventilator-induced lung injury (VILI) and worsen outcomes. A detailed understanding of the mechanisms that cause VILI will improve the treatment of ARDS and thus reduce mortality for a significant number of people.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Transition Award (R00)
Project #
5R00HL128944-04
Application #
9405899
Study Section
Special Emphasis Panel (NSS)
Program Officer
Reineck, Lora A
Project Start
2017-01-01
Project End
2019-12-31
Budget Start
2018-01-01
Budget End
2018-12-31
Support Year
4
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of Colorado Denver
Department
Engineering (All Types)
Type
Biomed Engr/Col Engr/Engr Sta
DUNS #
041096314
City
Aurora
State
CO
Country
United States
Zip Code
80045
Hamlington, Katharine L; Bates, Jason H T; Roy, Gregory S et al. (2018) Alveolar leak develops by a rich-get-richer process in ventilator-induced lung injury. PLoS One 13:e0193934
Hamlington, Katharine L; Smith, Bradford J; Dunn, Celia M et al. (2018) Linking lung function to structural damage of alveolar epithelium in ventilator-induced lung injury. Respir Physiol Neurobiol 255:22-29
Mori, Vitor; Smith, Bradford J; Suki, Bela et al. (2018) Linking Physiological Biomarkers of Ventilator-Induced Lung Injury to a Rich-Get-Richer Mechanism of Injury Progression. Ann Biomed Eng :
Knudsen, Lars; Lopez-Rodriguez, Elena; Berndt, Lennart et al. (2018) Alveolar Micromechanics in Bleomycin-induced Lung Injury. Am J Respir Cell Mol Biol 59:757-769
Bates, Jason H T; Smith, Bradford J (2018) Ventilator-induced lung injury and lung mechanics. Ann Transl Med 6:378
Broche, Ludovic; Perchiazzi, Gaetano; Porra, Liisa et al. (2017) Dynamic Mechanical Interactions Between Neighboring Airspaces Determine Cyclic Opening and Closure in Injured Lung. Crit Care Med 45:687-694
Smith, Bradford J; Bartolak-Suki, Elizabeth; Suki, Bela et al. (2017) Linking Ventilator Injury-Induced Leak across the Blood-Gas Barrier to Derangements in Murine Lung Function. Front Physiol 8:466
Smith, Bradford Julian (2016) Strain heterogeneity in the injured lung: cause or consequence? J Appl Physiol (1985) :jap.00818.2016