The purpose of this work is to modify the genomes of the mRNA viruses BMV (brome mosaic virus), Q beta bacteriophage and SBMV (southern bean mosaic virus) and their relatives in order to study the fundamental processes of viral genome replication, viral protein synthesis, virion assembly and their interrelationships. The immediate goals in making these modifications are both to alter virus-host interactions and to enable the construction of vectors for the introduction of new or alternative viral functions. Constructions will be made in which viral cDNAs and their RNA transcripts are intermediates in the infectious process in order to use the powerful techniques of recombinant DNA chemistry. Modifications will be introduced to further identify the proteins encoded in viral genomes and delineate their mechanism of action and to identify and characterize genomic regulatory sites.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI001466-35
Application #
3124086
Study Section
Virology Study Section (VR)
Project Start
1977-03-01
Project End
1992-06-30
Budget Start
1991-07-01
Budget End
1992-06-30
Support Year
35
Fiscal Year
1991
Total Cost
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
Other Domestic Higher Education
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Shaklee, P N; Miglietta, J J; Palmenberg, A C et al. (1988) Infectious positive- and negative-strand transcript RNAs from bacteriophage Q beta cDNA clones. Virology 163:209-13