Genetic, biochemical and biophysical methods are being used to examine a) the roles of bacterial and phage recombination systems in bacteriophage lambda recombination; b) the impact of DNA sequence non-homologies on recombination; c) the processing of base pair mismatches and DNA sequence non-homologies in artificially constructed heteroduplex molecules of lambda DNA; d) the role of recombination in post-replication repair, and e) the mechanism of Mu DNA integration following lytic infection.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI005388-25
Application #
3124240
Study Section
(MG)
Project Start
1977-12-01
Project End
1987-11-30
Budget Start
1986-12-01
Budget End
1987-11-30
Support Year
25
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Fox, M S; Radicella, J P; Yamamoto, K (1994) Some features of base pair mismatch repair and its role in the formation of genetic recombinants. Experientia 50:253-60
Radicella, J P; Clark, E A; Chen, S et al. (1993) Patch length of localized repair events: role of DNA polymerase I in mutY-dependent mismatch repair. J Bacteriol 175:7732-6
Kunes, S; Botstein, D; Fox, M S (1990) Synapsis-mediated fusion of free DNA ends forms inverted dimer plasmids in yeast. Genetics 124:67-80
Pearson, R K; Fox, M S (1988) Effects of DNA heterologies on bacteriophage lambda packaging. Genetics 118:5-12
Pearson, R K; Fox, M S (1988) Effects of DNA heterologies on bacteriophage lambda recombination. Genetics 118:13-9
Kunes, S; Ma, H; Overbye, K et al. (1987) Fine structure recombinational analysis of cloned genes using yeast transformation. Genetics 115:73-81
Orrego, C; Fox, M S (1985) On mutation fixation resulting from nitrosoguanidine-induced DNA damage in Escherichia coli K12 ada-. Mutat Res 146:185-9