Abstract

These studies are aimed at elucidation of 1) The pathway of biosynthesis of lipid A in Salmonella typhimurium; and 2) the molecular mechanisms of intermembrane translocation of lipopolysaccharide and phospholipid between inner and outer membrane. Genetic and biochemical approaches will be employed to analyze the pathway of lipid A synthesis. Conditional mutants in the pathway will be isolated, mapped and characterized biochemically with respect to the nature of mutant lipid A-related products formed and enzymatic reactions involved. Studies on translocation are aimed at identification of possible intermediate steps in lippopolysaccharide translocation - defective mutants and investigation of the relation of membrane topology to translocation of phospholipid.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI008650-17
Application #
3124437
Study Section
Microbial Physiology and Genetics Subcommittee 2 (MBC)
Project Start
1978-04-01
Project End
1988-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
17
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Connecticut
Department
Type
School of Medicine & Dentistry
DUNS #
City
Farmington
State
CT
Country
United States
Zip Code

  Publications

McGrath, B C; Osborn, M J (1991) Localization of the terminal steps of O-antigen synthesis in Salmonella typhimurium. J Bacteriol 173:649-54
Marino, P A; McGrath, B C; Osborn, M J (1991) Energy dependence of O-antigen synthesis in Salmonella typhimurium. J Bacteriol 173:3128-33
McGrath, B C; Osborn, M J (1991) Evidence for energy-dependent transposition of core lipopolysaccharide across the inner membrane of Salmonella typhimurium. J Bacteriol 173:3134-7
Marino, P A; Phan, K A; Osborn, M J (1985) Energy dependence of lipopolysaccharide translocation in Salmonella typhimurium. J Biol Chem 260:14965-70