Abstract

The long range goal of this proposal is to study the cell biology and biochemistry involved in the function of B cells as antigen presenting cells. We will focus our attention on two membrane glycoproteins that are known to be involved in this function, namely membrane immunogloblin and Ia-antigens. Furthermore we will attempt to gain information as to why activation of B cells seems to be important in allowing this cell to serve as an antigen presenting cell. As part of this investigation the mechanism by which Gamma-irradiation inhibits the antigen presenting cell capacity of these cells will be explored. We will also try and define other membrane glycoproteins or elements that might be involved in the antigen presenting capacity of B cells and in particular to evaluate the potential role of membrane proteases in the processing of certain antigens. Among the techniques that we plan to use to evaluate the role of Ig and membrane Iy in the antigen presenting capacity of B cells, we will purify these membrane glycoproteins and incorporate them into liposomes. These liposomes will either be utilized directly as antigen presenting units or they will be used as vehicles to incorporate these membrane molecules into other cell types in order to probe how they function in different cellular milieu.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
7R01AI009758-19
Application #
3124615
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1975-06-01
Project End
1990-05-31
Budget Start
1988-06-01
Budget End
1989-05-31
Support Year
19
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Cytel Corporation
Department
Type
DUNS #
City
San Diego
State
CA
Country
United States
Zip Code
92121

  Publications

Sette, A; Sidney, J; Albertson, M et al. (1990) A novel approach to the generation of high affinity class II-binding peptides. J Immunol 145:1809-13
Sette, A; Buus, S; Appella, E et al. (1990) Structural requirements for the interaction between class II MHC molecules and peptide antigens. Immunol Res 9:2-7
Demotz, S; Grey, H M; Sette, A (1990) The minimal number of class II MHC-antigen complexes needed for T cell activation. Science 249:1028-30
Buus, S; Sette, A; Colon, S M et al. (1988) Autologous peptides constitutively occupy the antigen binding site on Ia. Science 242:1045-7
Krieger, J; Jenis, D M; Chesnut, R W et al. (1988) Studies on the capacity of intact cells and purified Ia from different B cell sources to function in antigen presentation to T cells. J Immunol 140:388-94
Grammer, S F; Ishioka, G Y; Chesnut, R W (1988) Studies on the capacity of B cells as well as T cells to serve as accessory cells for the activation of herpes simplex virus-specific cytolytic T cells. J Immunol 140:2016-22
Bekoff, M C; Cole, B C; Grey, H M (1987) Studies on the mechanism of stimulation of T cells by the Mycoplasma arthritidis-derived mitogen. Role of class II IE molecules. J Immunol 139:3189-94
Krieger, J I; Chesnut, R W; Grey, H M (1986) Capacity of B cells to function as stimulators of a primary mixed leukocyte reaction. J Immunol 137:3117-23
Buus, S; Colon, S; Smith, C et al. (1986) Interaction between a ""processed"" ovalbumin peptide and Ia molecules. Proc Natl Acad Sci U S A 83:3968-71
Coeshott, C M; Chesnut, R W; Kubo, R T et al. (1986) Ia-specific mixed leukocyte reactive T cell hybridomas: analysis of their specificity by using purified class II MHC molecules in synthetic membrane system. J Immunol 136:2832-8

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