Abstract

Major progress during the last twelve months has been in the amino acid sequence analysis of the anti-arsonate hybridomas that have been generated in A/J mice. The primary structural analysis has revealed an unexpected degree of heterogeneity and the relationship between these structures and idiotypy are under current investigation. During the last twelve months, twelve different publications have resulted from these kinds of studies, all published in referred journals. More recently, we have utilized funds from this grant to establish T cell hybrids in the arsonate system, and while separate funding for this project has just been requested, it was under the auspices of this grant and the projects derived therein that this important piece of work was initiated. In addition to the above, this grant has supported primary structural analysis of human immunoglobulin variable regions and three papers have been submitted for publication concerning the relationship of the cross-idiotypic specificity among the mixed cryoglobulins and their primary structure.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Project (R01)
Project #
5R01AI012127-12
Application #
3125117
Study Section
Allergy and Immunology Study Section (ALY)
Project Start
1979-05-01
Project End
1987-08-31
Budget Start
1986-09-01
Budget End
1987-08-31
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Texas Sw Medical Center Dallas
Department
Type
Overall Medical
DUNS #
City
Dallas
State
TX
Country
United States
Zip Code
75390

  Publications

Jackson, Stephen M; Harp, Natessa; Patel, Darshna et al. (2009) Key developmental transitions in human germinal center B cells are revealed by differential CD45RB expression. Blood 113:3999-4007
Jackson, Stephen M; Harp, Natessa; Patel, Darshna et al. (2007) CD45RO enriches for activated, highly mutated human germinal center B cells. Blood 110:3917-25
Jackson, S M; Harp, N; Patel, D et al. (2007) CD45RO: a marker for BCR-mediated selection. Scand J Immunol 66:249-60
Rahman, Negar S; Godderz, LeAnn J; Stray, Stephen J et al. (2006) DNA cleavage of a cryptic recombination signal sequence by RAG1 and RAG2. Implications for partial V(H) gene replacement. J Biol Chem 281:12370-80
Jackson, Stephen M; Capra, J Donald (2005) IgH V-region sequence does not predict the survival fate of human germinal center B cells. J Immunol 174:2805-13
Kolar, Grant R; Yokota, Takafumi; Rossi, Maria Isabel D et al. (2004) Human fetal, cord blood, and adult lymphocyte progenitors have similar potential for generating B cells with a diverse immunoglobulin repertoire. Blood 104:2981-7
Rodgers, William; Jordan, Stephen J; Capra, J Donald (2002) Transient association of Ku with nuclear substrates characterized using fluorescence photobleaching. J Immunol 168:2348-55
Carayannopoulos, M O; Potter, K N; Li, Y et al. (2000) Evidence that human immunoglobulin M rheumatoid factors can Be derived from the natural autoantibody pool and undergo an antigen driven immune response in which somatically mutated rheumatoid factors have lower affinities for immunoglobulin G Fc than thei Scand J Immunol 51:327-36
Frazer, J K; Jackson, D G; Gaillard, J P et al. (2000) Identification of centerin: a novel human germinal center B cell-restricted serpin. Eur J Immunol 30:3039-48
Tuaillon, N; Capra, J D (2000) Evidence that terminal deoxynucleotidyltransferase expression plays a role in Ig heavy chain gene segment utilization. J Immunol 164:6387-97

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